...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Vaccine >Level of protection of chickens against highly pathogenic H5 avian influenza virus with Newcastle disease virus based live attenuated vector vaccine depends on homology of H5 sequence between vaccine and challenge virus.
【24h】

Level of protection of chickens against highly pathogenic H5 avian influenza virus with Newcastle disease virus based live attenuated vector vaccine depends on homology of H5 sequence between vaccine and challenge virus.

机译：基于新城疫病毒的减毒活疫苗对鸡的高致病性H5禽流感病毒的保护水平取决于疫苗与攻击病毒之间H5序列的同源性。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Vaccination of poultry against avian influenza is of high priority, in particular after the dramatic spread of subtype H5N1 in Asia, Africa and Europe. Newcastle disease virus (NDV) has been developed as a vector for the expression of the main immunogen of avian influenza virus, hemagglutinin (HA). An NDV vector based vaccine has several advantages. It allows easy serological differentiation between infected and vaccinated animals by the detection of antibodies against non-HA influenza proteins. Moreover, it can be administered easily to large numbers of animals by spray or drinking water. We recently showed that chickens could be protected against infection with highly pathogenic avian influenza virus (HPAIV) A/chicken/Italy/8/98 (H5N2) after immunization with a recombinant Newcastle disease virus, NDVH5m, which expresses the homologous hemagglutinin. Here, we describe that immunization with NDVH5m conferred only partial protection against lethal infection with heterologous HPAIV A/duck/Vietnam/TG24-01/05 (H5N1). Comparison of amino acid sequences of both H5 proteins showed only 93.6% amino acid identity. Therefore, a new NDV recombinant (NDVH5Vm) was generated which expresses the H5 protein of HPAIV A/chicken/Vietnam/P41/05 (H5N1). This recombinant virus protected chickens against lethal infection with HPAIV H5N1 (Vietnam) already after one immunization. Our data thus show that application of a vector-based vaccine in the control of influenza may require adaptation of the vaccine to currently circulating viruses.
机译：尤其是在亚洲，非洲和欧洲广泛传播H5N1亚型禽流感之后，对禽流感进行疫苗接种是当务之急。已开发出新城疫病毒（NDV）作为表达禽流感病毒主要免疫原血凝素（HA）的载体。基于NDV载体的疫苗具有多个优点。通过检测针对非HA流感蛋白的抗体，可以轻松区分受感染和接种疫苗的动物。而且，可以通过喷雾或饮用水将其容易地施用于大量动物。我们最近表明，用表达同源血凝素的重组新城疫病毒NDVH5m免疫后，可以保护鸡免受高致病性禽流感病毒（HPAIV）A /鸡/意大利/ 8/98（H5N2）的感染。在这里，我们描述了用NDVH5m进行的免疫接种只能部分保护免受异源HPAIV A /鸭/越南/ TG24-01 / 05（H5N1）致死性感染。两种H5蛋白的氨基酸序列比较仅显示93.6％的氨基酸同一性。因此，产生了新的NDV重组体（NDVH5Vm），其表达HPAIV A /鸡/越南/越南/ P41 / 05（H5N1）的H5蛋白。一次免疫后，这种重组病毒已保护鸡免受HPAIV H5N1（越南）的致命感染。因此，我们的数据表明，基于载体的疫苗在控制流感中的应用可能需要使疫苗适应当前正在传播的病毒。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号