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Cytokine mRNAs in the nasal-associated lymphoid tissue during influenzavirus infection and nasal vaccination

机译:流感病毒感染和鼻疫苗接种期间鼻相关淋巴组织中的细胞因子mRNA

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Intranasal immunization with a current inactivated influenza vaccine together with an adjuvant (cholera toxin B subunit supplemented with a trace amount of whole toxin, CTB*) was confirmed in BALB/c mice to mimic influenza virus (A/PR/8/34, H1N1) infection with respect to mucosal IgA antibody responses, in which IgA antibody-forming cell responses in the nasal-associated lymphoid tissue (NALT) were involved with a peak around 7 days after infection or vaccination. Next, the expression of various cytokine mRNAs in the NALT was compared in mice either infected with viruses or immunized with CTB*-combined vaccine, to examine Th cell and cytokine regulation of mucosal IgA. antibody responses. In infected mice, strong IL-2, weak IL-4, strong IL-6 and strong IFN-gamma mRNA expressions were induced during early days of infection; especially, IFN-gamma mRNA was expressed by both CD4(+) and CD8(+) T cells around 7 days after infection. In mice given CTB*-combined vaccine, weak IL-2, strong IL-4, strong IL-6 and weak IFN-gamma mRNA expressions were induced during early days of vaccination; especially, IL-4 mRNA was expressed by CD4+ T cells. Thus, IL-6 mRNAs were expressed strongly in both infected and vaccinated mice. The IFN-gamma-rich cytokine mRNA profiles in the infected mice were reflected upon serum IgG2a-rich Ab responses, while the IL-4-rich profiles in the vaccinated mice were reflected upon the IgG1-rich Ab responses. Thus, influenza virus infection and CTB*-combined nasal vaccine induced Th1 dominant and Th2 dominant cytokine profiles, respectively, while the similarity of mucosal IgA antibody responses between infection and vaccination could be explained by the appearance of IL-6 mRNAs.
机译:在BALB / c小鼠中证实了用目前的灭活流感疫苗和佐剂(霍乱毒素B亚基补充了痕量全毒素,CTB *)进行鼻内免疫,可模拟流感病毒(A / PR / 8/34,H1N1 )关于粘膜IgA抗体反应的感染,其中与鼻相关的淋巴样组织(NALT)中的IgA抗体形成细胞反应涉及感染或疫苗接种后7天左右的高峰。接下来,比较感染病毒或用CTB *组合疫苗免疫的小鼠中NALT中各种细胞因子mRNA的表达,以检查Th细胞和细胞因子对粘膜IgA的调节。抗体反应。在感染的小鼠中,在感染的早期诱导了强IL-2,弱IL-4,强IL-6和强IFN-γmRNA表达。特别是,感染后7天左右,CD4(+)和CD8(+)T细胞均表达了IFN-γmRNA。在接种CTB *组合疫苗的小鼠中,疫苗接种的早期诱导了IL-2,IL-4,IL-6和IFN-γmRNA的弱表达。特别是,IL-4 mRNA在CD4 + T细胞中表达。因此,IL-6 mRNA在感染和接种疫苗的小鼠中均强烈表达。感染的小鼠中富含IFN-γ的细胞因子mRNA谱反映在富含IgG2a的血清中Ab应答,而接种的小鼠中富含IL-4的谱反映在富含IgG1的Ab应答中。因此,流感病毒感染和CTB *组合鼻疫苗分别诱导了Th1显性和Th2显性的细胞因子谱,而感染和疫苗接种之间粘膜IgA抗体反应的相似性可以通过IL-6 mRNA的出现来解释。

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