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首页> 外文期刊>Vaccine >Nanogram quantities of a DNA vaccine protect rainbow trout fry againstheterologous strains of infectious hematopoietic necrosis virus
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Nanogram quantities of a DNA vaccine protect rainbow trout fry againstheterologous strains of infectious hematopoietic necrosis virus

机译：脱氧核糖核酸疫苗的纳米级数量可以保护虹鳟鱼苗免受传染性造血坏死病毒的异源株

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摘要

The efficacy of a DNA Vaccine containing the glycoprotein gene of infectious hematopoietic necrosis virus (IHNV), a rhabdovirus affecting trout and salmon, was investigated. The minimal dose of vaccine required, the protection against heterologous strains, and the titers of neutralizing antibodies produced were used to evaluate the potential of the vaccine as a control pharmaceutical, Results indicated that a single dose of as little as 1-10 ng of Vaccine protected rainbow trout fry against waterborne challenge by IHNV. An optimal dose of 100 ng per fish was selected to assure strong protection under various conditions. Neutralizing antibody titers were detected in fish vaccinated with concentrations of DNA ranging from 5 to 0.01 mu g. Furthermore, the DNA vaccine protected fish against a broad range of viral strains from different geographic locations, including isolates from France and Japan, suggesting that the Vaccine could be used worldwide. A single dose of this DNA vaccine induced protection in fish at a lower dose than is usually reported in mammalian DNA vaccine studies. Published by Elsevier Science Ltd.
机译：研究了含有传染性造血坏死病毒（IHNV）糖蛋白基因（影响鳟鱼和鲑鱼的弹状病毒）的DNA疫苗的功效。使用所需的最小剂量的疫苗，针对异源菌株的保护以及产生的中和抗体的效价来评估疫苗作为对照药物的潜力。结果表明，单剂量疫苗的剂量仅为1-10 ng保护虹鳟鱼苗免受IHNV的水上挑战。选择了每条鱼100 ng的最佳剂量，以确保在各种条件下都具有强大的保护作用。在接种了5至0.01μgDNA浓度的鱼中检测到中和抗体滴度。此外，DNA疫苗可保护鱼类免受来自不同地理位置的多种病毒株的侵害，包括来自法国和日本的分离株，这表明该疫苗可在全球范围内使用。单剂量的这种DNA疫苗以比哺乳动物DNA疫苗研究中通常报道的剂量低的剂量在鱼类中引起保护作用。由Elsevier Science Ltd.发布

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