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首页> 外文期刊>Vaccine >Phenotypic changes in the Japanese encephalitis virus after one passage in Neuro-2a cells: Generation of attenuated strains of the virus
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Phenotypic changes in the Japanese encephalitis virus after one passage in Neuro-2a cells: Generation of attenuated strains of the virus

机译：在Neuro-2a细胞中传代一次后，日本脑炎病毒的表型变化：病毒减毒株的产生

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摘要

Live attenuated strains of the Japanese encephalitis (JE) virus are known to form in various cultured cells. In this study, we selected attenuated JE virus variants by passing the parent virus strains once through Neuro-2a cells, showing that the selection intensity ranged 2-1000-fold after infection for 3 d. The selection of attenuated variants appeared in association with mutations on the envelope (E) glycoprotein. This is likely determined by the differential binding abilities of specific E proteins with highly sulfated glycosaminoglycans on the cell surface. A plaque-purification method showed that Neuro-2a-selected variants were usually less neurovirulent, leading to a longer survival time of intracerebrally inoculated mice. Specific selected variants (mostly with the small-plaque phenotype) were shown to be more efficient at replication in Vero cells and less virulent, particularly those with substitution of the E-138 (Glu -> Lys) residue. As a result, most, if not all, low-virulent variantswere generated in a relatively short time, all which have the potential to be live-attenuated vaccine candidates of the JE virus.
机译：已知在各种培养细胞中会形成日本脑炎（JE）病毒减毒活株。在这项研究中，我们通过使亲代病毒株一次通过Neuro-2a细胞选择了减毒的JE病毒变体，表明感染3天后选择强度范围是2-1000倍。减毒变体的选择与包膜（E）糖蛋白上的突变相关。这很可能是由特定E蛋白与细胞表面高度硫酸化的糖胺聚糖的不同结合能力决定的。噬菌斑纯化方法显示，Neuro-2a选择的变体通常具有较低的神经毒性，从而导致脑内接种小鼠的存活时间更长。特定选择的变体（大多数具有小噬斑表型）显示出在Vero细胞中复制更有效，且毒性较低，尤其是那些被E-138（Glu-> Lys）残基取代的变体。结果，大多数（即使不是全部）低毒力变异体在相对较短的时间内产生，所有这些变异体都有可能成为减毒的JE病毒候选疫苗。

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