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首页> 外文期刊>Vaccine >Codelivery of adjuvants at the primary immunization site is essential for evoking a robust immune response to neoglycoconjugates.
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Codelivery of adjuvants at the primary immunization site is essential for evoking a robust immune response to neoglycoconjugates.

机译：佐剂在初次免疫位点的代码传递对于引起对新糖缀合物的强大免疫反应至关重要。

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A series of nonformulated adjuvants, i.e. Quil-A, MPL, DDA, CpG and Alum were codelivered with a synthetic branched tetrasaccharide fragment corresponding to Streptococcus pneumoniae type 14 polysaccharide (Pn14PS) conjugated to CRM197 in order to investigate antibody- and cell-mediated immune responses in a mouse model. The first immunization was performed intracutaneously in the presence of adjuvants. Booster injections which were given without adjuvant dramatically enhanced and expanded the immune response from IgM to IgG1 and other IgG subclasses. Codelivery of the neoglycoconjugate with CpG or Alum had no additional effect over vaccine in saline, and elicited mainly IgG1 antibodies against Pn14PS. CpG even has a modest suppressive effect on the antibody response. Codelivery of the neoglycoconjugate with Quil-A, MPL, DDA alone or in combination resulted in both IgG1, IgG2a, IgG2b and IgG3 antibodies. Quil-A alone or in combination with MPL induced systemic IL-5 and IL-6 6 h after primary immunization. This adjuvant combination also increased CD4/CD8 T cells ratio in lymph nodes and peripheral blood on day 1 after immunization. Seven days later, the ratios in blood and lymph nodes had returned to normal. In conclusion, codelivery of Quil-A alone or in combination with MPL had the most dramatic effect on antibody- and cell-mediated immune response to neoglycoconjugate of Pn14PS.
机译：一系列非配方佐剂，即Quil-A，MPL，DDA，CpG和Alum，与对应于与CRM 197共轭的肺炎链球菌14型多糖（Pn14PS）的合成分支支链四糖片段共编码为了研究小鼠模型中抗体和细胞介导的免疫反应。第一次免疫是在佐剂存在下经皮内进行的。没有佐剂的加强注射大大增强并扩大了从IgM到IgG1和其他IgG亚类的免疫反应。新糖缀合物与CpG或明矾的共递送对盐水中的疫苗没有附加作用，并且主要引起针对Pn14PS的IgG1抗体。 CpG甚至对抗体应答具有适度的抑制作用。新糖缀合物单独或与Quil-A，MPL，DDA的共传递会产生IgG1，IgG2a，IgG2b和IgG3抗体。初次免疫后6小时，单独或与MPL组合使用Quil-A诱导全身性IL-5和IL-6。免疫后第1天，这种佐剂组合还可增加淋巴结和外周血中CD4 / CD8 T细胞的比例。七天后，血液和淋巴结中的比例恢复正常。总之，单独或与MPL组合使用Quil-A的代码传递对Pn14PS新糖缀合物的抗体和细胞介导的免疫反应具有最显着的影响。

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