A heterologous prime-boost regime using DNA and recombinant vaccinia virus expressing the Leishmania infantum P36/LACK antigen protects BALB/c mice from cutaneous leishmaniasis

Gonzalo RM; del Real G; Rodriguez JR; Rodriguez D; Heljasvaara R

首页> 外文期刊>Vaccine >A heterologous prime-boost regime using DNA and recombinant vaccinia virus expressing the Leishmania infantum P36/LACK antigen protects BALB/c mice from cutaneous leishmaniasis

【24h】

A heterologous prime-boost regime using DNA and recombinant vaccinia virus expressing the Leishmania infantum P36/LACK antigen protects BALB/c mice from cutaneous leishmaniasis

机译：使用表达婴儿利什曼原虫P36 / LACK抗原的DNA和重组牛痘病毒的异源初免-加强疗法可保护BALB / c小鼠免于皮肤利什曼病

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

A heterologous prime-boost vaccination with DNA vectors and vaccinia virus recombinants (VVr) has been shown to enhance specific cellular immune responses and to elicit significant protection against pathogens in animal models. In this study, we have analyzed, in the leishmaniasis cutaneous murine model, the effectiveness of this prime-boost strategy by immunizing with a DNA vector followed by boost with a VVr expressing the same Leishmania infantum P36/LACK antigen. After DNA priming, and VVr boost, we challenged susceptible BALB/c mice with live L. major promastigotes, and examined the increase in footpad lesion size and parasite load in drainin g lymph nodes. Compared to controls, we observed reduction of up to 70% in lesion size and 1000-fold in parasite load. DNA prime-VVr boost before challenge elicited a Thl type immune response in spleen cells from immunized animals. This DNA/VVr vaccination approach could be of utility in the prophylaxis against leishmaniasis.

机译：用DNA载体和牛痘病毒重组体（VVr）进行异源初免-加强免疫接种已显示出可增强特异性细胞免疫反应并在动物模型中引起针对病原体的显着保护作用。在这项研究中，我们已经在利什曼病皮肤鼠模型中分析了这种初免-加强策略的有效性，方法是先用DNA载体免疫，再用表达相同婴儿利什曼原虫P36 / LACK抗原的VVr加强免疫。 DNA引发和VVr增强后，我们用活的主要乳头前鞭毛虫攻击了易感的BALB / c小鼠，并检查了足垫病变大小的增加和引流淋巴结中的寄生虫负荷。与对照组相比，我们观察到病灶大小减少了多达70％，寄生虫负荷减少了1000倍。攻击前的DNAprime-VVr增强在来自免疫动物的脾细胞中引发了Thl型免疫反应。这种DNA / VVr疫苗接种方法可用于预防利什曼病。

著录项

来源
《Vaccine》 |2002年第8期|共6页
作者
Gonzalo RM; del Real G; Rodriguez JR; Rodriguez D; Heljasvaara R;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类卫生标准、卫生检查、医药管理;
关键词
Leishmaniasis; Leishmania infantum; Recombinant vaccinia virus; Cd8(+) t-cells; Murine leishmaniasis; Interferon-gamma; Vaccination; Immunity; Malaria; Interleukin-4; Immunization; Progression; Resolution;

机译：利什曼病;婴儿利什曼原虫;重组牛痘病毒;Cd8（+）t细胞;鼠利什曼病;干扰素-γ;疫苗接种;免疫;疟疾;白介素-4;免疫;进展;解决方案;

相似文献

外文文献
中文文献
专利

1. A heterologous prime-boost regime using DNA and recombinant vaccinia virus expressing the Leishmania infantum P36/LACK antigen protects BALB/c mice from cutaneous leishmaniasis [J] . Gonzalo RM, del Real G, Rodriguez JR, Vaccine . 2002,第7a8期

机译：使用表达婴儿利什曼原虫P36 / LACK抗原的DNA和重组牛痘病毒的异源初免-加强疗法可保护BALB / c小鼠免于皮肤利什曼病
2. Protective immune response against cutaneous leishmaniasis by prime/booster immunization regimens with vaccinia virus recombinants expressing Leishmania infantum p36/LACK and IL-12 in combination with purified p36. [J] . Gonzalo RM, Rodriguez JR, Rodriguez D, Microbes and infection . 2001,第9期

机译：通过表达婴儿利什曼原虫p36 / LACK和IL-12的牛痘病毒重组体与纯化的p36结合的初免/加强免疫方案，针对皮肤利什曼病的保护性免疫应答。
3. The combination of DNA vectors expressing IL-12 + IL-18 elicits high protective immune response against cutaneous leishmaniasis after priming with DNA-p36/LACK and the cytokines, followed by a booster with a vaccinia virus recombinant expressing p36/ [J] . Esher Tapia, Eva Perez-Jimenez, Laura Lopez-Fuertes, Microbes and infection . 2003,第2期

机译：表达IL-12 + IL-18的DNA载体的组合引发DNA-p36 / LACK和细胞因子引发的皮肤利什曼病的高度保护性免疫应答，随后是表达p36 / L36的牛痘病毒重组的加强免疫。
4. The Toxicity Evaluation of Recombinant Replicating Vaccinia Virus Vaccine in BALB/c Mice [C] . Sanglong WANG, Iimei CAO, Yin LIU, The 5th Session of the 13th annual meeting, CAST; Interantional conference on chemicals risk assessment of environmental hazard and human health. . 2011

机译：重组复制痘苗病毒疫苗对BALB / c小鼠的毒性评估
5. Applicability of vaccinia virus as cloning and expression vector for bacterial genes: Mice immune responses to vaccinia virus expressing Brucella abortus and Listeria monocytogenes antigens. [D] . Baloglu, Simge. 2006

机译：牛痘病毒作为细菌基因的克隆和表达载体的适用性：小鼠对表达布鲁氏菌流产单核细胞增生李斯特菌抗原的牛痘病毒的免疫反应。
6. Antigenicity of Leishmania-Activated C-Kinase Antigen (LACK) in Human Peripheral Blood Mononuclear Cells and Protective Effect of Prime-Boost Vaccination With pCI-neo-LACK Plus Attenuated LACK-Expressing Vaccinia Viruses in Hamsters [O] . Laura Fernández, Eugenia Carrillo, Lucas Sánchez-Sampedro, -1

机译：利什曼原虫激活的C激酶抗原（LACK）在人外周血单核细胞中的抗原性以及pCI-neo-LACK加减毒的表达LACK的痘苗病毒在仓鼠中初免接种疫苗的保护作用。
7. Heterologous prime-boost vaccination with a non-replicative vaccinia recombinant vector expressing LACK confers protection against canine visceral leishmaniasis with a predominant Th1-specific immune response [O] . Ramos, Irene, Alonso, Ana, Marcen, Jose Miguel, 2008

机译：用表达LACK的非复制牛痘重组载体进行异源初免-加强免疫接种可预防犬内脏利什曼病并具有Th1特异性免疫应答

相关主题

A heterologous prime-boost regime using DNA and recombinant vaccinia virus expressing the Leishmania infantum P36/LACK antigen protects BALB/c mice from cutaneous leishmaniasis

摘要

著录项

相似文献

相关主题

期刊订阅