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首页> 外文期刊>Vaccine >A randomized, placebo-controlled trial of subcutaneous administration of GM-CSF as a vaccine adjuvant: effect on cellular and humoral immune responses
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A randomized, placebo-controlled trial of subcutaneous administration of GM-CSF as a vaccine adjuvant: effect on cellular and humoral immune responses

机译：皮下注射GM-CSF作为疫苗佐剂的随机，安慰剂对照试验：对细胞和体液免疫反应的影响

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Thirty healthy volunteers were randomly assigned to receive either a single subcutaneous injection of GM-CSF or placebo at the time of vaccination with tetanus and diptheria toxoid (Td), influenza and hepatitis A vaccines. Humoral response was measured by weekly serum samples assayed for antibodies to tetanus toxoid (TT), influenza and hepatitis A; while cellular response to TT was determined by measuring IL-2 expression in T-cells following in vitro exposure to TT antigen using a flow cytometric assay. It was hypothesized that (1) GM-CSF would augment immune response and (2) that the frequencies of TT responsive T-cells in the blood would predict Immoral responses. The administration of subcutaneous GM-CSF as an adjuvant at the time of vaccination did not augment the antibody responses to influenza or hepatitis A in normal volunteers when compared to placebo. Subjects who received GM-CSF had statistically significant lower increases in anti-tetanus antibodies than placebo recipients. Immunization with TT resulted in an increase in the frequency of antigen responsive T-cells in the blood over time. The frequencies of TT responsive T-cells in baseline blood samples were correlated with baseline anti-tetanus antibody titers, but humoral and cellular responses were not correlated following vaccination. Recipients of GM-CSF did not develop significantly higher numbers of TT responsive T-cells after vaccination compared to recipients who received placebo.
机译：30名健康志愿者被随机分配接受破伤风和白喉类毒素（Td），流感和甲型肝炎疫苗接种时皮下注射GM-CSF或安慰剂。通过每周血清样品检测破伤风类毒素（TT），流行性感冒和甲型肝炎的抗体来测量体液反应。而通过使用流式细胞仪测定体外暴露于TT抗原后测量T细胞中IL-2的表达来确定对TT的细胞反应。据推测，（1）GM-CSF将增强免疫反应，（2）血液中TT反应性T细胞的频率将预测不道德反应。与安慰剂相比，在接种疫苗时皮下注射GM-CSF作为佐剂在正常志愿者中并未增强针对流感或甲型肝炎的抗体反应。统计学上，接受GM-CSF的受试者抗破伤风抗体的增加显着低于安慰剂接受者。随着时间的推移，TT免疫导致血液中抗原反应性T细胞的频率增加。基线血样中TT反应性T细胞的频率与基线抗破伤风抗体滴度相关，但接种疫苗后体液和细胞反应均不相关。与接受安慰剂的接受者相比，接种疫苗后GM-CSF的接受者没有发育出更高数量的TT反应性T细胞。

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