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Three types of immunotherapics against pythiosis insidiosi developed and evaluated

机译：开发和评估了三种针对化脓性尿崩症的免疫疗法

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摘要

Pythiosis is a granulomatous disease of horses, cattle, dogs, cats and humans identified in tropical and subtropical areas and caused by Pythium insidiosum. a zoosporic fungus. Experimental models of pythiosis in naturally infected species have not yet been reported but, rabbits may be inoculated with zoospores as an experimental model for studying the disease. The present study evaluates the efficacy of three different of immunotherapics in the rabbit model. Approximately 17,500 zoospores of oomycete P. insidiosum (CBS 101555 strain) were inoculated in each animal to generate the disease. Immunotherapics were produced from vortexed or sonicated cultures of the same strain. Four groups of five animals were employed: group 1, placebo; group 2, sonicated immunotherapic; group 3, mixed immunotherapic; and group 4, vortexed immunotherapic. All rabbits were inoculated with viable zoospores one month before administration of the immunotherapics. Eight doses of immunotherapic or placebo were used in each animal with a 14 day interval between injections. Rabbits receiving the vortexed immunotherapic were most effectively protected (P < 0.05), showing a decrease in the area of coastal nodules due to Pythiosis insidiosum by 71.8% after 26 weeks of evaluation. Moreover, two animals in this group showed complete remission of the infection at the end of the 26 weeks. In contrast to these findings, rabbits given the sonicated immunotherapic did not show any protection and had an increase of 211.8% in the size of lesions. This failure of sonicated immunotherapic may reflect denaturation of protective antigens due to the sonication method.
机译：Pythiosis是一种在热带和亚热带地区发现的马，牛，狗，猫和人的肉芽肿病，是由腐霉病引起的。动物孢子菌。尚未报道自然感染物种中的硫烟病实验模型，但可以用游动孢子接种兔子作为研究该病的实验模型。本研究评估了三种不同的免疫疗法在兔模型中的功效。在每只动物中接种大约17,500个卵菌的印度隐孢子虫游动孢子（CBS 101555菌株）以产生该疾病。从同一菌株的涡旋或超声处理培养物中产生免疫治疗剂。使用四组，每组五只动物：第一组，安慰剂;第二组。第2组，超声免疫治疗;第3组，混合免疫疗法;第4组，涡旋免疫治疗。在给予免疫治疗前一个月，所有兔子都接种了活的游动孢子。每只动物使用八剂免疫治疗剂或安慰剂，两次注射之间间隔14天。接受涡旋免疫治疗的兔子得到了最有效的保护（P <0.05），在经过26周的评估后，由于Indidisum引起的沿海结节面积减少了71.8％。此外，该组中的两只动物在26周结束时表现出感染的完全缓解。与这些发现相反，给予超声免疫治疗的兔子未显示任何保护作用，病变面积增加了211.8％。超声免疫治疗的这种失败可能反映了由于超声方法引起的保护性抗原的变性。

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