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首页> 外文期刊>Vaccine >Characterization of virulence of Leptospira isolates in a hamster model.
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Characterization of virulence of Leptospira isolates in a hamster model.

机译：在仓鼠模型中钩端螺旋体分离物的毒性特征。

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摘要

Effort has been made to identify protective antigens in order to develop a recombinant vaccine against leptospirosis. Several attempts failed to conclusively demonstrate efficacy of vaccine candidates due to the lack of an appropriate model of lethal leptospirosis. The purposes of our study were: (i) to test the virulence of leptospiral isolates from Brazil, which are representative of important serogroups that cause disease in humans and animals; and (ii) to standardize the lethal dose 50% (LD(50)) for each of the virulent strains using a hamster (Mesocricetus auratus) model. Five of seven Brazilian isolates induced lethality in a hamster model, with inocula lower than 200 leptospires. Histopathological examination of infected animals showed typical lesions found in both natural and experimental leptospirosis. Results described here demonstrated the potential use of Brazilian isolates as highly virulent strains in challenge experiments using hamster as an appropriate animal model for leptospirosis. Furthermore these strains may be useful in heterologous challenge studies which aim to evaluate cross-protective responses induced by sub-unit vaccine candidates.
机译：为了开发抗钩端螺旋体病的重组疫苗，已努力鉴定保护性抗原。由于缺乏合适的致死性钩端螺旋体病模型，多次尝试未能最终证明候选疫苗的功效。我们的研究目的是：（i）测试来自巴西的钩端螺旋体分离株的毒力，这些分离株代表了引起人和动物疾病的重要血清群; （ii）使用仓鼠（Mesocricetus auratus）模型将每种毒性菌株的致死剂量标准化为50％（LD（50））。七个巴西分离株中有五个在仓鼠模型中诱发了致死性，接种量低于200个钩端螺旋体。感染动物的组织病理学检查显示在自然和实验性钩端螺旋体病中均发现典型的病变。此处描述的结果表明，使用仓鼠作为钩端螺旋体病的合适动物模型，在挑战性实验中，巴西分离株可作为高毒力菌株使用。此外，这些菌株在异源攻击研究中可能有用，该研究旨在评估由亚单位疫苗候选物诱导的交叉保护反应。

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