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首页> 外文期刊>Vaccine >Comparison of different prime-boost regimes with DNA and recombinant Orf virus based vaccines expressing glycoprotein D of pseudorabies virus in pigs.
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Comparison of different prime-boost regimes with DNA and recombinant Orf virus based vaccines expressing glycoprotein D of pseudorabies virus in pigs.

机译：用猪中表达伪狂犬病病毒糖蛋白D的DNA和重组基于Orf病毒的疫苗比较不同的初免-加强方案。

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摘要

Both DNA and Orf virus (ORFV; Parapox virus) based vaccines have shown promise as alternatives for conventional vaccines in pigs against pseudorabies virus (PRV) infection causing Aujeszky's disease. In the present study we evaluated the efficacy of different prime-boost regimes in pigs in terms of immunogenicity and protection against challenge infection with PRV. The different prime-boost regimes consisted of the homologous prime-boost regimes (DNA followed by DNA or ORFV followed by ORFV) and the heterologous prime-boost regimes (DNA followed by ORFV and ORFV followed by DNA), all based on glycoprotein D (gD) of PRV. Moreover, we compared the efficacy of the different prime-boost regimes with the efficacy of a conventional modified live vaccine (MLV). The different prime-boost regimes resulted in different levels of immunity and protection against challenge infection. Most effective was the regime of priming with DNA vaccine followed by boosting with the ORFV based vaccine. This regime resulted in strong antibody responses, comparable to the antibody responses obtained after prime-boost vaccination with a conventional MLV vaccine. Also with regard to protection, the prime DNA-boost ORFV regime performed better than the other prime-boost regimes. This study demonstrates the potential of a heterologous prime-boost vaccination strategy against PRV based on a single antigen, and that in the natural host, the pig.
机译：基于DNA和Orf病毒（ORFV;副痘病毒）的疫苗都已显示出有望作为猪中常规疫苗的替代品，以对抗引起狂犬病的伪狂犬病病毒（PRV）感染。在本研究中，我们从免疫原性和针对PRV激发感染的防护方面评估了不同初免-加强方案在猪中的功效。不同的初免-加强方案由同源的初免-加强方案（DNA，然后是DNA或ORFV，然后是ORFV）和异源的初-加强方案（DNA，然后是ORFV和ORFV，然后是DNA）组成，所有这些都基于糖蛋白D（ gD）。此外，我们将不同的初免-加强方案的功效与常规改良活疫苗（MLV）的功效进行了比较。不同的初免-加强方案导致了不同水平的免疫力和针对攻击性感染的保护。最有效的方法是先使用DNA疫苗进行初次接种，然后再使用基于ORFV的疫苗进行加强免疫。这种方案导致了强大的抗体反应，与常规MLV疫苗初免-加强疫苗接种后获得的抗体反应相当。同样在保护方面，主要的DNA增强型ORFV方案比其他主要的增强型方案表现更好。这项研究证明了针对PRV的异源初免-加强疫苗接种策略以及基于天然宿主猪的策略的潜力。

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