Identification of HLA-DR4-restricted T-cell epitope on MPT51 protein, a major secreted protein derived from Mycobacterium tuberculosis using MPT51 overlapping peptides screening and DNA vaccination.

Wang LiXin; Nagata T.; Tsujimura K.; Uchijima M.; Seto S.; Koide Y.

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Identification of HLA-DR4-restricted T-cell epitope on MPT51 protein, a major secreted protein derived from Mycobacterium tuberculosis using MPT51 overlapping peptides screening and DNA vaccination.

机译：使用MPT51重叠肽段筛选和DNA疫苗接种，鉴定MPT51蛋白上的HLA-DR4限制性T细胞表位，MPT51是结核分枝杆菌的主要分泌蛋白。

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摘要

We identified a novel HLA-DR4-restricted CD4+ T-cell epitope on a secreted antigen of Mycobacterium tuberculosis, MPT51, in 004149-MM HLA-DR4-transgenic mice which express HLA-DRB1*0401, but not murine MHC class II molecules. The mice were immunized with plasmid DNA encoding MPT51 using gene gun and interferon (IFN)- gamma production from the immune splenocytes was analyzed. In response to overlapping synthetic peptides covering the mature MPT51 sequence, only one peptide, p191-210, stimulated the splenocytes to produce IFN- gamma . Further analysis using flow cytometry and computer-assisted algorithm, ProPred, narrowed down the region of CD4+ T-cell epitope to p191-202. The CD4+ T-cell epitope would be feasible for vaccine design against tuberculosis as well as for analysis of MPT51-specific T-cells in M. tuberculosis infection.

机译：我们在表达HLA-DRB1 * 0401的004149-MM HLA-DR4-转基因小鼠中鉴定了结核分枝杆菌MPT51分泌抗原上的一种新型HLA-DR4限制性CD4 + T细胞表位。不是鼠类MHC II类分子。使用基因枪用编码MPT51的质粒DNA免疫小鼠，并分析免疫脾细胞中干扰素（IFN）-γ的产生。对于覆盖成熟MPT51序列的重叠合成肽，只有一种肽p191-210刺激脾细胞产生IFN-γ。使用流式细胞仪和计算机辅助算法ProPred进行的进一步分析将CD4 + T细胞表位的区域缩小到p191-202。 CD4 + T细胞表位对于设计抗结核疫苗以及分析M中MPT51特异性T细胞将是可行的。结核感染。

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《Vaccine》 |2010年第8期|共6页
作者
Wang LiXin; Nagata T.; Tsujimura K.; Uchijima M.; Seto S.; Koide Y.;
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正文语种 eng
中图分类医学免疫学;
关键词
bacterial antigens; bacterial proteins; CD4+ lymphocytes; cytokines; DNA; DNA vaccines; epitopes; human leukocyte antigens; immune response; immunization; interferon; laboratory animals; splenocytes; T lymphocytes; tuberculosis; vaccination; mice; Mycobacterium tuberculosis;

机译：细菌抗原;细菌蛋白;CD4 +淋巴细胞;细胞因子;DNA;DNA疫苗;表位;人白细胞抗原;免疫反应;免疫;干扰素;实验室动物;脾细胞;T淋巴细胞;结核病;疫苗接种;小鼠;结核分枝杆菌;

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1. Identification of HLA-DR4-restricted T-cell epitope on MPT51 protein, a major secreted protein derived from Mycobacterium tuberculosis using MPT51 overlapping peptides screening and DNA vaccination. [J] . Wang LiXin, Nagata T., Tsujimura K., Vaccine . 2010,第8期

机译：使用MPT51重叠肽段筛选和DNA疫苗接种，鉴定MPT51蛋白上的HLA-DR4限制性T细胞表位，MPT51是结核分枝杆菌的主要分泌蛋白。
2. Identification of Murine H2-Dd- and H2-Ab-Restricted T-Cell Epitopes on a Novel Protective Antigen, MPT51, of Mycobacterium tuberculosis [J] . Mina Suzuki, Taiki Aoshi, Toshi Nagata, Infection and immunity . 2004,第7期

机译：结核分枝杆菌的新型保护性抗原MPT51上的小鼠H2-Dd和H2-Ab限制性T细胞表位的鉴定
3. Functional insights from a comparative study on the dynamics of Antigen85 proteins and MPT51 from Mycobacterium tuberculosis [J] . Sundar Shobana, Annaraj David, Selvan Anitha, Journal of molecular modeling . 2015,第12期

机译：通过对结核分枝杆菌Antigen85蛋白和MPT51动力学进行比较研究获得的功能见解
4. Identifying Immunogenic CD4+ T-cell Epitopes of Myeloid Cell Leukemia 1 Using Overlapping 20-mer Peptides Spanning the Whole Protein [C] . Joshua S. Woodworth, Else M. Agger, Paul R. Hansen American Peptide Symposium . 2015

机译：使用跨越整个蛋白质的重叠20-MEL肽鉴定骨髓细胞白血病1的免疫原性CD4 + T细胞表位
5. Dynamics of peptides bound to class I major histocompatibility complex proteins: Implications for T-cell receptor recognition in the immune response [D] . Insaidoo, Francis Kobina 2010

机译：与I类主要组织相容性复杂蛋白结合的肽的动力学：免疫应答中T细胞受体识别的意义
6. Identification of an HLA-A*0201-Restricted T-Cell Epitope on the MPT51 Protein a Major Secreted Protein Derived from Mycobacterium tuberculosis by MPT51 Overlapping Peptide Screening [O] . Taiki Aoshi, Toshi Nagata, Mina Suzuki, 2008

机译：通过MPT51重叠肽段筛选鉴定MPT51蛋白上的HLA-A * 0201限制性T细胞表位MPT51是结核分枝杆菌的主要分泌蛋白。
7. Identification of an HLA-A*0201-Restricted T-Cell Epitope on the MPT51 Protein, a Major Secreted Protein Derived from Mycobacterium tuberculosis, by MPT51 Overlapping Peptide Screening▿ [O] . Aoshi, Taiki, Nagata, Toshi, Suzuki, Mina, 2008

机译：通过MPT51重叠肽段筛选鉴定MPT51蛋白（一种来自结核分枝杆菌的主要分泌蛋白）上的HLA-A * 0201限制性T细胞表位▿
8. Identification of a Bacteria Using Phylogenetic Relationships Revealed by MS/MS Sequencing of Tryptic Peptides Derived From Cellular Proteins [R] . Dworzanski, J. P. , Wick, H. , Snyder, A. P. , 2004

机译：利用系统发育关系鉴定细菌，通过细胞蛋白衍生的胰蛋白酶消化肽的ms / ms测序揭示

Identification of HLA-DR4-restricted T-cell epitope on MPT51 protein, a major secreted protein derived from Mycobacterium tuberculosis using MPT51 overlapping peptides screening and DNA vaccination.

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