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首页> 外文期刊>Vaccine >Adjuvantive effects of anti-4-1BB agonist Ab and 4-1BBL DNA for a HIV-1 Gag DNA vaccine: different effects on cellular and humoral immunity.
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Adjuvantive effects of anti-4-1BB agonist Ab and 4-1BBL DNA for a HIV-1 Gag DNA vaccine: different effects on cellular and humoral immunity.

机译：抗4-1BB激动剂Ab和4-1BBL DNA对HIV-1 Gag DNA疫苗的辅助作用：对细胞和体液免疫的不同作用。

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摘要

Plasmid DNA immunizations induce low levels but a broad spectrum of cellular and humoral immune responses. Here, we investigate the potential of co-stimulation through 4-1BB as an adjuvant for a HIV-1 DNA vaccine in mice. We designed plasmid DNAs expressing either the membrane bound or soluble form of 4-1BBL, and compared with the agonistic anti-4-1BB Ab for their ability to adjuvant the Gag DNA vaccine. Both, anti-4-1BB agonistic Ab as well as 4-1BBL DNA enhanced the Gag-specific cellular immune responses. However, in complete contrast to the agonistic Ab that suppressed humoral immunity to Gag, 4-1BBL DNA adjuvanted vaccines enhanced Gag-specific IgG responses. Importantly, the expression of Gag and 4-1BBL from the same plasmid was critical for the adjuvant activity. Collectively, our data suggest that for a HIV-1 vaccine where both antigen-specific cellular and humoral immunity are desirable, 4-1BBL expressed by a DNA vaccine is a superior adjuvant than anti-4-1BB agonistic Ab.
机译：质粒DNA免疫可诱导低水平的免疫反应，但可引起广泛的细胞和体液免疫反应。在这里，我们调查了通过4-1BB作为小鼠HIV-1 DNA疫苗佐剂的共刺激潜能。我们设计了表达膜结合或可溶形式的4-1BBL的质粒DNA，并与激动性的抗4-1BB Ab相比，它们具有佐剂Gag DNA疫苗的能力。抗4-1BB激动剂Ab和4-1BBL DNA均可增强Gag特异性细胞免疫应答。但是，与抑制针对Gag的体液免疫的激动性Ab完全相反，4-1BBL DNA佐剂疫苗增强了Gag特异性IgG反应。重要的是，来自同一质粒的Gag和4-1BBL的表达对于佐剂活性至关重要。总体而言，我们的数据表明，对于同时需要抗原特异性细胞免疫和体液免疫的HIV-1疫苗，DNA疫苗表达的4-1BBL比抗4-1BB激动剂Ab具有更好的佐剂。

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