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首页> 外文期刊>Vaccine >pcDNA-IL-12 vaccination blocks eosinophilic inflammation but not airway hyperresponsiveness following murine Toxocara canis infection
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pcDNA-IL-12 vaccination blocks eosinophilic inflammation but not airway hyperresponsiveness following murine Toxocara canis infection

机译：pcDNA-IL-12疫苗接种可阻止鼠弓形虫感染后嗜酸性粒细胞炎症，但不能阻止气道高反应性

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We have investigated the effect of pcDNA3-CpG and pcDNA-IL-12, delivered by intradermal gene gun administration, on the blood/lung eosinophilia, airway hyperresponsiveness as well as the immune response in a murine model of toxocariasis. Our results demonstrated that pcDNA-IL-12 but not pcDNA3-CpG vaccination led to a persistent lower blood/bronchoalveolar eosinophilia following Toxocara canis infection, as pcDNA3-CpG led only to an early transient blockage of eosinophil transmigration into bronchoalveolar fluid following T. canis infection. Prominent Type-1 immune response was pointed out as the hallmark of T. canis infection following pcDNA-IL-12 vaccination. Outstanding IFN-gamma/IL-4 ratio besides low levels of IgG1 with subsequent high IgG2a/IgG1 ratio further characterized a Type-1 polarized immunological profile in pcDNA-IL-12-vaccinated animals. Nevertheless, only pcDNA3-CpG was able to prevent airway hyperresponsiveness induced by T. canis infection. The persistent airway hyperresponsiveness observed in pcDNA-IL-12-vaccinated animals demonstrated that the airway constriction involved other immunological mediator than those blocked by pcDNA-IL-12. Together, these data indicated that pcDNA-IL-12 and pcDNA3-CpG vaccines have distinct therapeutic benefits regarding the eosinophilic inflammation/airway hyperresponsiveness triggered by T. canis infection, suggesting their possible use in further combined therapeutic interventions.
机译：我们已经研究了通过皮内基因枪给药传递的pcDNA3-CpG和pcDNA-IL-12对血液/肺嗜酸性粒细胞增多，气道高反应性以及在弓形虫病小鼠模型中的免疫反应的影响。我们的研究结果表明，pcDNA-IL-12疫苗接种但未接种pcDNA3-CpG疫苗，导致犬弓形虫感染后持续存在较低的血液/支气管肺泡嗜酸性粒细胞，因为pcDNA3-CpG仅导致早期嗜热性粒细胞迁移到犬T.colon过渡到支气管肺泡液的早期阻断。感染。指出了突出的1型免疫应答是pcDNA-IL-12疫苗接种后犬圆弧菌感染的标志。除了低水平的IgG1和随后的高水平的IgG2a / IgG1比率外，杰出的IFN-γ/ IL-4比率进一步表征了接种pcDNA-IL-12的动物的Type-1极化免疫学特征。然而，只有pcDNA3-CpG能够预防犬圆弧菌感染引起的气道高反应性。在接种了pcDNA-IL-12的动物中观察到的持续气道高反应性表明，气道收缩还涉及其他免疫学介质，而不是被pcDNA-IL-12阻断的介质。总之，这些数据表明，pcDNA-IL-12和pcDNA3-CpG疫苗在犬毛球菌感染引发的嗜酸性炎症/气道高反应性方面具有独特的治疗优势，表明它们可能在进一步的联合治疗干预中使用。

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