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首页> 外文期刊>Vaccine >Gene gun-mediate DNA vaccination against foot-and-mouth disease virus
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Gene gun-mediate DNA vaccination against foot-and-mouth disease virus

机译：基因枪介导的针对口蹄疫病毒的DNA疫苗

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Foot-and-mouth disease (FMD) is one of the most dangerous diseases of cloven-hoofed animals and is a constant threat in the Middle-East and other regions throughout the world despite intensive Vaccination programs. In this work, we describe the ability of FMDV expression constructs to protect pigs from FMDV challenge when used as a vaccine. The construct consists of encephalomyocarditis virus (EMCV) internal ribosome entry site (IRES), the entire P1 and 2A together with 3CD sequences, all in the same reading frame. Another plasmid that was tested, carries the serotype O1 (G) VPI, Asia1 VP1 and O1 (G) 3C. Between each of the genes the 3C cleavage sequences were inserted. All constructs carried the cytomegalo virus (CMV) promoter. Using immunofluorescent and immunoblot techniques, we could show the expression and processing of viral proteins. Following the application of FMDV expression constructs into pigs skin by 'Gene Gun', pigs were partially protected from FMDV challenge.
机译：口蹄疫（FMD）是丁香蹄类动物最危险的疾病之一，尽管实施了严格的疫苗接种计划，但在中东和世界其他地区仍是持续威胁。在这项工作中，我们描述了当用作疫苗时，FMDV表达构建体保护猪免受FMDV攻击的能力。该构建体由脑心肌炎病毒（EMCV）内部核糖体进入位点（IRES），整个P1和2A以及3CD序列组成，均位于同一阅读框中。另一个经过测试的质粒带有血清型O1（G）VPI，Asia1 VP1和O1（G）3C。在每个基因之间插入3C切割序列。所有构建体均携带巨细胞病毒（CMV）启动子。使用免疫荧光和免疫印迹技术，我们可以显示病毒蛋白的表达和加工。在通过“基因枪”将FMDV表达构建体应用于猪皮后，猪受到了FMDV攻击的部分保护。

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