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Protection induced by intramuscular immunization with DNA vaccines of pseudorabies in mice, rabbits and piglets

机译：伪狂犬病DNA疫苗肌肉内免疫对小鼠，兔和仔猪的保护作用

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Glycoprotein gene gB, gC and gD of pseudorabies virus (PrV) strain Ea, which was isolated locally in Wuhan, were cloned from the viral genome DNA and expressed in vitro controlled by the major immediately-early promotor/enhancer of HCMV. In the presented paper, Balb/c mice, rabbits and piglets were vaccinated intramuscularly two times at 2-week interval with those eukaryotic expression plasmid pcDB, pcDC and pcDD, respectively. The animals injected with pcDB, pcDC, pcDD or mix DNA developed anti-PrV antibodies. Neutralizing antibody titers obtained 2-5 log(2), 2 weeks after the second vaccination. Cellular immune responses were also detected by lymphoproliferation assay and cytotoxic T lymphocyte (CTL) activity assay in all groups vaccinated with DNA. Immune responses elicited by DNA vaccines provided protections with different degrees against lethal dose PrV challenge. In mice, protections induced by pcDC, pcDD or mix DNA were 100%, similar to that by inactivated vaccine. Protections were more than 50% induced by pcDC, pcDD or mix DNA in rabbits. Protections induced by pcDB were the lowest among DNA immunization in mice or rabbits. However, pcDB could elicit the higher cellular responses in rabbits or piglets, In piglets, body temperatures of animals injected with pcDB, pcDC, pcDD or mix DNA did not change significantly after challenge with 2 x 10(5) pfu of PrV strain Ea, and the means daily growth post-challenge of those animals were higher than those injected with inactivated vaccine or parental plasmid. Neither DNA vaccines nor inactivated vaccine could prevent or delay virus excretion after challenge. Our experiments in experimental animals and natural hosts suggested the efficiency and potential application of DNA vaccines for pseudorabies in pigs.
机译：从病毒基因组DNA中克隆了在武汉本地分离的伪狂犬病病毒（PrV）菌株Ea的糖蛋白基因gB，gC和gD，并在HCMV的主要早期启动子/增强子的控制下体外表达。在本文中，将Balb / c小鼠，兔和小猪以2周的间隔分别肌肉注射两次真核表达质粒pcDB，pcDC和pcDD。注射了pcDB，pcDC，pcDD或混合DNA的动物产生了抗PrV抗体。第二次疫苗接种后2周，中和抗体滴度为2-5 log（2）。在所有接种了DNA的组中，还通过淋巴增殖试验和细胞毒性T淋巴细胞（CTL）活性试验检测了细胞免疫应答。 DNA疫苗引起的免疫反应在不同程度上提供了针对致命剂量PrV攻击的保护作用。在小鼠中，由pcDC，pcDD或混合DNA诱导的保护作用为100％，与灭活疫苗相似。 pcDC，pcDD或混合DNA对家兔的保护作用超过50％。在小鼠或兔子的DNA免疫中，pcDB诱导的保护作用最低。但是，pcDB可能在兔或仔猪中引发更高的细胞反应。在仔猪中，注射2x 10（5）pfu PrV菌株Ea攻击后，注射pcDB，pcDC，pcDD或混合DNA的动物的体温没有明显变化，并且这些动物在攻击后的日均生长速度高于注射灭活疫苗或亲本质粒的动物。 DNA疫苗和灭活疫苗都无法预防或延缓攻击后病毒的排泄。我们在实验动物和自然宿主中进行的实验表明，猪伪狂犬病DNA疫苗的效率和潜在应用。

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