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首页> 外文期刊>Vaccine >CD4(+) and CD8(+) mediated cellular immune response to recombinant influenza nucleoprotein
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CD4(+) and CD8(+) mediated cellular immune response to recombinant influenza nucleoprotein

机译：CD4（+）和CD8（+）介导的重组流感核蛋白的细胞免疫反应

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摘要

The stimulatory properties of soluble recombinant influenza nucleoprotein (NP) on purified CD4(+) and CD8(+) T cells from young and elderly individuals were studied. Recombinant influenza NP failed to induce proliferation of resting CD4(+) and CD8(+) T cells in the absence of IL-2. Addition of small amounts of IL-2, however, led to strong proliferation of resting CD4(+) and CD8(+) T cells from young and elderly donors. NP-reactive CD4(+) and CD8(+) T cell lines from both age groups grew equally well under long-term culture conditions. T cell lines raised to live influenza virus could recognize recombinant influenza NP and showed a substantial proliferative response. Stimulation of CD8(+) T cells is presumably due to cross-presentation, as EBV-transformed MHC class I-positive cell lines, which are incapable of antigen processing, stimulated live influenza virus-reactive CD8(+) T cell lines when loaded with NP-derived immunodominant peptides but not following loading with the whole NP molecule. Vaccines containing recombinant influenza NP might confer cross-protective immunity and could therefore be especially useful in cases of major epidemics or pandemics.
机译：研究了可溶性重组流感核蛋白（NP）对来自年轻人和老年人的纯化CD4（+）和CD8（+）T细胞的刺激特性。在没有IL-2的情况下，重组流感NP不能诱导静止的CD4（+）和CD8（+）T细胞增殖。但是，添加少量的IL-2会导致年轻和老年供体的静息CD4（+）和CD8（+）T细胞强烈增殖。在长期培养条件下，两个年龄段的NP反应性CD4（+）和CD8（+）T细胞系均生长良好。产生活流感病毒的T细胞系可以识别重组流感NP，并显示出实质性的增殖反应。推测CD8（+）T细胞的刺激是由于交叉表达，因为加载后无法进行抗原加工的EBV转化的MHC I类阳性细胞系刺激了活流感病毒反应性CD8（+）T细胞系使用NP衍生的免疫优势肽，但不加载整个NP分子。含有重组流感NP的疫苗可能具有交叉保护性免疫，因此在重大流行病或大流行病的情况下尤其有用。

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