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首页> 外文期刊>Vaccine >DNA Immunization of mice with a plasmid encoding Neisseria gonorrhea PorB protein by intramuscular injection and epidermal particle bombardment
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DNA Immunization of mice with a plasmid encoding Neisseria gonorrhea PorB protein by intramuscular injection and epidermal particle bombardment

机译：肌肉注射和表皮粒子轰击法对编码淋病奈瑟氏菌PorB蛋白的质粒的小鼠进行DNA免疫

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Immunogenicity of a DNA vaccine encoding PorB from Neisseria gonorrhoeae strain FA 1090 was analyzed in BALB/C mice immunized by intramuscular needle injection or epidermal gene gun bombardment. Both delivery routes generated measurable specific antibodies although the gene gun response was slower. Antibody isotypes were indicative of Th2 activation following gene gun immunization and of Th1 activation following intramuscular injection. In both immunization protocols, boosting with either renatured recombinant (rr) PorB protein or PorB expressed from Venezuelan equine encephalitis virus replicon particles (VRPs) significantly increased anti-PorB antibody levels. Boosting with rrPorB protein had little effect on antibody isotypes, while boosting with VRPs expressing PorB-enhanced a Th1 type response. Whole cell binding experiments showed that a portion of the antibodies recognized the surface of the homologous N. gonorrhoeae strain. Serum from groups with high antibody levels showed some opsonization of the homologous strain using human neutrophils. These results showed the potential of DNA vaccination for the purpose of priming an antibody response against PorB of N. gonorrhoeae. When combined with a protein or VRP boost, DNA priming resulted in high-titer and long-lasting responses. Based on different prime-boost protocols, we could polarize immune responses to predominantly Th1 or Th2, which should enable future studies of the types of immune responses that are protective in mouse models of gonorrhea. (C) 2003 Elsevier Ltd. All rights reserved
机译：在通过肌肉内注射或表皮基因枪轰击免疫的BALB / C小鼠中分析了淋病奈瑟氏球菌FA 1090编码PorB的DNA疫苗的免疫原性。尽管基因枪反应较慢，但两种递送途径均产生可测量的特异性抗体。抗体同种型指示基因枪免疫后Th2激活和肌肉注射后Th1激活。在两种免疫方案中，用复性的重组（rr）PorB蛋白或从委内瑞拉马脑炎病毒复制子颗粒（VRPs）表达的PorB加强免疫，均可显着提高抗PorB抗体水平。用rrPorB蛋白加强免疫对抗体同种型几乎没有影响，而用表达PorB的VRP加强免疫增强了Th1型应答。全细胞结合实验表明部分抗体识别同源淋病奈瑟氏球菌菌株的表面。具有高抗体水平的人群的血清显示使用人嗜中性粒细胞对同源菌株进行调理作用。这些结果表明了DNA疫苗接种的潜力，以引发针对淋病奈瑟氏球体的PorB的抗体应答。当与蛋白质或VRP增强剂结合使用时，DNA引发可导致高滴度和持久的应答。基于不同的初免-升压方案，我们可以使免疫反应主要针对Th1或Th2极化，这应能进一步研究对淋病小鼠模型具有保护作用的免疫反应类型。 （C）2003 Elsevier Ltd.保留所有权利

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