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首页> 外文期刊>Vaccine >Protective efficacy of a fully recombinant plague vaccine in the guinea pig
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Protective efficacy of a fully recombinant plague vaccine in the guinea pig

机译：完全重组鼠疫疫苗对豚鼠的保护功效

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摘要

A fully recombinant sub-unit vaccine comprising the protein antigens rF1 + rV has been demonstrated to protect immunised guinea pigs against exposure to 10(5) colony-forming units (CFU) of virulent Yersinia pestis. Additionally, IgG purified from rF1 + rV-immunised guinea pig serum, protected the mouse by passive immunisation against challenge with Y pestis whereas IgG purified from the serum of guinea pigs immunised with a licensed killed whole cell (KWC) vaccine for plague, protected less well. Guinea pigs immunised with the licensed killed whole cell vaccine developed an IgG titre for fraction I (F I) but not for V antigen. The differential in protection conferred on the mouse by passive immunisation with guinea pig IgG, was abrogated by the use of IgG purified from guinea pigs immunised with killed whole cell vaccine supplemented with V antigen. These findings indicate that the reduced efficacy of the licensed killed whole cell vaccine formulation previously observed in the mouse can be attributed to lack of the V antigen. Cross-protection of the mouse with guinea pig IgG suggests that the recognition of neutralising epitopes in the F1 and V proteins is conserved between these two species.
机译：已证明包含蛋白质抗原rF1 + rV的完全重组亚单位疫苗可保护免疫的豚鼠免于暴露于10（5）有毒力耶尔森氏菌的菌落形成单位（CFU）。此外，从rF1 + rV免疫的豚鼠血清中纯化的IgG通过被动免疫保护小鼠免受Y鼠疫杆菌的攻击，而从经许可的鼠疫全细胞（KWC）疫苗免疫鼠疫的豚鼠血清中纯化的IgG保护较少好。用许可的灭活全细胞疫苗免疫的豚鼠对I部分（F I）产生了IgG效价，但对V抗原却没有产生IgG效价。通过使用豚鼠IgG纯化的IgG可以消除小鼠通过豚鼠IgG被动免疫而产生的保护差异，而IgG是从用补充有V抗原的灭活全细胞疫苗免疫的豚鼠中纯化得到的。这些发现表明，先前在小鼠中观察到的许可的杀死的全细胞疫苗制剂的功效降低可归因于缺乏V抗原。小鼠与豚鼠IgG的交叉保护表明，在这两个物种之间，F1和V蛋白中和表位的识别是保守的。

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