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Identification of novel immunogenic proteins of Shigella flexneri 2a by proteomic methodologies

机译:蛋白质组学方法鉴定弗氏志贺氏菌2a的新型免疫原性蛋白

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摘要

Shigella spp. are one of the most important etiological factors for people who are living in developing countries and travelers to tropical countries. High priority has been given by the World Health Organization to the development of vaccines to control Shigellosis caused by these bacteria. However, information regarding to profile of immunogenic proteins of Shigella is not available now. In the present study, sub-immunoproteomics was applied to screen novel immunogenic proteins which could be reacted with antisera produced by challenge of a whole bacterium. Our results indicated that 13 immunogens were identified, in which seven proteins and six proteins from outer membrane and soluble proteome, respectively. Of the 13 proteins, 12 showed to be novel immunogens. These results suggest that immunoproteomics can greatly improve the chances of identification and result in discovery of novel immunogenic proteins.
机译:志贺氏菌对于居住在发展中国家的人和前往热带国家的旅行者来说,这是最重要的病因之一。世界卫生组织已高度重视开发疫苗来控制由这些细菌引起的志贺氏菌病。但是,有关志贺氏菌免疫原性蛋白谱的信息现在不可用。在本研究中,亚免疫组学被用于筛选新型免疫原性蛋白质,该蛋白质可与通过挑战整个细菌而产生的抗血清反应。我们的结果表明,已鉴定出13种免疫原,其中7种蛋白质和6种蛋白质分别来自外膜和可溶性蛋白质组。在这13种蛋白质中,有12种是新的免疫原。这些结果表明免疫蛋白质组学可以大大提高鉴定的机会,并导致发现新的免疫原性蛋白质。

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