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首页> 外文期刊>Vaccine >Immunogenicity and anti-Burkholderia pseudomallei activity in Balb/c mice immunized with plasmid DNA encoding flagellin
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Immunogenicity and anti-Burkholderia pseudomallei activity in Balb/c mice immunized with plasmid DNA encoding flagellin

机译：用编码鞭毛蛋白的质粒DNA免疫的Balb / c小鼠的免疫原性和抗伯克霍尔德菌假mallei活性

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Plasmid DNA encoding the flagella protein (flagellin) was used as a vaccination candidate for the evaluation of its immunogenicity and for protection against infection with Burkholderia pseudomallei. Firstly, flagellin encoding plasmid DNA was injected into Balb/c mice intramuscularly and this elicited both a humoral and a cellular immune response. Total IgG production and the clonal expansion of the spleen cells increased in response to flagellin. The IgG subclass response exhibited a dominance of IgG2a over IgG1 in the sera. In addition, IFN-gamma-secreting cells in the spleen were substantially increased. Furthermore, the anti-B. pseudomallei activity of the peritoneal exudate cells was evaluated by a Transwell tissue-culture plate system where the macrophage-activating related cytokines in upper chamber were allowed to cross the plate's membrane and stimulate the activation of peritoneal exudate cells in lower chamber. Our results indicated that the activated peritoneal exudate cells were able to restrict the growth of B. pseudomallei in vitro. Indeed, subsequent intravenous challenge of the vaccinated Balb/c mice with 10(5)CFU of B. pseudomallei resulted in the number of bacterial cells detected in liver and/or spleen being significantly reduced in the flagellin plasmid DNA vaccinated mice. At 7 days subsequent to infection of B. pseudomallei, 5/6 (83%) of flagellin plasmid DNA vaccinated mice had survived. We suggest that plasmid DNA-encoding flagellin might be useful as a potential immunization route for the future development of a vaccine against melioidosis in related animals.
机译：编码鞭毛蛋白（鞭毛蛋白）的质粒DNA被用作疫苗候选者，以评估其免疫原性并保护免受假伯克霍尔德氏菌感染。首先，将鞭毛蛋白编码质粒DNA肌肉注射到Balb / c小鼠中，这引起体液和细胞免疫反应。响应鞭毛蛋白，总IgG产生和脾细胞的克隆扩增增加。 IgG亚类应答在血清中显示出IgG2a优于IgG1。另外，脾脏中IFN-γ分泌细胞显着增加。再者，抗乙。通过Transwell组织培养板系统评估腹膜渗出液的假苹果酸活性，在该系统中，允许上腔室中的巨噬细胞激活相关细胞因子穿过板膜并刺激下腔室中的腹膜渗出细胞的激活。我们的结果表明，活化的腹膜渗出细胞能够在体外限制假苹果芽孢杆菌的生长。实际上，随后用10（5）CFU的假苹果芽孢杆菌对接种的Balb / c小鼠进行静脉内攻击，导致在鞭毛蛋白质粒DNA接种的小鼠中，肝脏和/或脾脏中检测到的细菌细胞数量大大减少。感染假疟原虫后第7天，接种鞭毛蛋白质粒DNA的小鼠中有5/6（83％）存活。我们建议，编码质粒DNA的鞭毛蛋白可能作为潜在的免疫途径，用于将来在相关动物中开发针对类鼻疽病的疫苗。

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