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首页> 外文期刊>Vaccine >Differentiation of convalescent animals from those vaccinated against foot-and-mouth disease by a peptide ELISA
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Differentiation of convalescent animals from those vaccinated against foot-and-mouth disease by a peptide ELISA

机译：通过肽酶联免疫吸附法（ELISA）将恢复期动物与针对口蹄疫疫苗接种的动物区分开

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摘要

Continuous antigenic determinants within the amino acid sequences of the conserved non-structural region containing proteins 2C and 3ABC of foot-and-mouth disease virus which can distinguish between the sera from vaccinated and infected animals wereidentified. An ELISA based on a 3B peptide gave a positive reaction with sera from cattle, pigs, sheep and guineapigs infected with all seven serotypes of the virus, but not with sera from vaccinated animals. In experiments with cattle and pigs to determine the duration of the antibody response, positive reactions were obtained as late as one year after infection. The advantages of using peptides from the non-structural viral proteins instead of recombinant proteins for differentiating vaccinated frominfected animals include their specificity, non-reactivity with antibodies against host cell-derived proteins (e.g. E. coli and insect cell proteins), and their ease of preparation.
机译：确定了在保守的非结构性区域的氨基酸序列中的连续抗原决定簇，该保守性非决定性区域包含口蹄疫病毒的蛋白质2C和3ABC，可以区分疫苗和感染动物的血清。基于3B肽的ELISA与感染了该病毒的所有7种血清型的牛，猪，绵羊和几内亚的血清产生了阳性反应，但未与接种动物的血清产生阳性反应。在用牛和猪进行的实验中确定抗体反应的持续时间，直到感染后一年才获得阳性反应。使用非结构病毒蛋白的肽代替重组蛋白来区分从感染动物中接种疫苗的优势包括其特异性，与抗宿主细胞衍生蛋白（例如大肠杆菌和昆虫细胞蛋白）的抗体无反应性以及其易用性准备。

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