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首页> 外文期刊>Vaccine >Recombinant virus-like particles as a carrier of B- and T-cell epitopes of hepatitis C virus (HCV)
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Recombinant virus-like particles as a carrier of B- and T-cell epitopes of hepatitis C virus (HCV)

机译：重组病毒样颗粒作为丙型肝炎病毒（HCV）B和T细胞表位的载体

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摘要

The major aim of the project was the development of virus-like particles (VLP) displaying B- and T-cell epitopes of hepatitis C virus (HCV) proteins. To this end, hepatitis B virus core (HBc) particles were used as a carrier of HCV epitopes. Fragments of HCV genes encoding core (aa 98) and NS3 (aa 155) proteins were fused to the 3' terminus of the truncated HBV core gene. All recombinant plasmids led to relatively high levels of expression of chimeric proteins in E. coli, which resulted in the formation of complete "mature" VLP. Chimeric HBc/HCV VLPs were purified by combination of gel filtration and sucrose gradient centrifugation, and used for immunogenicity studies in mice. All variants of hybrid particles induced high humoral and cellular responses to HBcAg. Immunization with the HBc/HCV core particles led to relatively low antibody and T-cell proliferative responses to HCV core epitopes. The HBc/HCV NS3 particles were able to induce high levels of anti-NS3 antibodies in the absence of proliferative responses to HCV epitopes. Thus, the results of the current study have demonstrated the principal possibility of using VLP on the basis of HBcAg for creation of a new type of HCV-specific immunogen.
机译：该项目的主要目的是开发展示丙型肝炎病毒（HCV）蛋白B细胞和T细胞表位的病毒样颗粒（VLP）。为此，乙肝病毒核心（HBc）颗粒被用作HCV表位的载体。将编码核心（aa 98）和NS3（aa 155）蛋白的HCV基因片段融合到截短的HBV核心基因的3'末端。所有重组质粒导致嵌合蛋白在大肠杆菌中的表达水平较高，从而导致形成完整的“成熟” VLP。嵌合的HBc / HCV VLP通过凝胶过滤和蔗糖梯度离心相结合纯化，并用于小鼠的免疫原性研究。杂种颗粒的所有变体均引起对HBcAg的高体液和细胞反应。 HBc / HCV核心颗粒免疫后，对HCV核心表位的抗体和T细胞增殖反应相对较低。在不存在对HCV表位的增殖反应的情况下，HBc / HCV NS3颗粒能够诱导高水平的抗NS3抗体。因此，本研究的结果证明了使用基于HBcAg的VLP产生新型HCV特异性免疫原的主要可能性。

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