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首页> 外文期刊>Vaccine >Oral delivery of lipid-encapsulated Mycobacterium bovis BCG extends survival of the bacillus in vivo and induces a long-term protective immune response against tuberculosis
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Oral delivery of lipid-encapsulated Mycobacterium bovis BCG extends survival of the bacillus in vivo and induces a long-term protective immune response against tuberculosis

机译：脂质包裹的牛分枝杆菌卡介苗的口服递送可延长体内细菌的存活率，并诱导针对肺结核的长期保护性免疫应答

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The success of oral-route vaccination using Mycobacterium bovis bacille Calmette-Guerin (BCG) relies on delivery of live, actively metabolising bacilli to confer protection. Here, we describe that lipid-microencapsulation can extend the in vivo survival of bacilli when fed to mice, and can induce a long-lasting protective immune response. Feeding mice with lipid-encapsulated BCG (L-BCG) resulted in greater recovery of viable BCG bacilli from the mesenteric lymph nodes (MLN) compared to mice fed non-encapsulated BCG. A time-course study indicated persistence of viable BCG bacilli in MLN up to 30 weeks post-vaccination, similar to the duration of viable BCG recovery from the spleen following subcutaneous vaccination. The persistence of viable bacilli in the MLN of L-BCG mice invoked long-lasting systemic cell-mediated immune reactivity, with responses similar to those observed in subcutaneously-vaccinated mice. Further, L-BCG-vaccinated mice showed a high degree of protection against aerogenic challenge with virulent M. bovis at 30 weeks post-vaccination, with significant reductions in lung and spleen pathogen burdens. This study identifies that lipid-encapsulation of live BCG bacilli can facilitate increased in vivo survival and immunogenicity of the vaccine in orally-vaccinated mice, and highlights protection via this route for up to 7 months post-immunisation.
机译：使用牛分枝杆菌杆菌Calmette-Guerin（BCG）进行口服途径疫苗接种的成功取决于递送活的，积极代谢的细菌以提供保护。在这里，我们描述了脂质微囊化可以在喂给小鼠时延长杆菌的体内存活，并可以诱导持久的保护性免疫应答。饲喂脂质包封的BCG（L-BCG）的小鼠，与饲喂未灌封的BCG的小鼠相比，可从肠系膜淋巴结（MLN）回收更多的活性BCG杆菌。一项时程研究表明，接种疫苗后30周内，MLN中仍存在有活性的BCG细菌，这与皮下接种疫苗后从脾脏中恢复出有效的BCG的持续时间相似。活细菌在L-BCG小鼠的MLN中的持久性引起了持久的系统性细胞介导的免疫反应，其反应与皮下接种的小鼠相似。此外，接种L-BCG的小鼠在接种后30周时，对强力牛分枝杆菌表现出高度的抵抗气源性攻击的能力，大大降低了肺和脾病原体的负担。这项研究表明，对活的BCG细菌进行脂质包封可以促进口服疫苗接种的小鼠体内存活率的提高和疫苗的免疫原性，并强调了在免疫后长达7个月通过这种途径提供的保护。

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