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Comparison of different adjuvants of protein and DNA vaccination for the prophylaxis of IgE antibody formation

机译：蛋白质和DNA疫苗接种不同佐剂预防IgE抗体形成的比较

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A high-molecular-weight mite allergen Der f11 that was hardly purified for immunotherapy was used to develop the DNA vaccine pDf11. We have shown that vaccination of mice with pDf11 induces Th1 responses characterized by suppression of IgE responses. In the present study, effects of different adjuvants on pDf11 were first studied. Mice receiving pDf11 +/- CpG, bestatin, and bupivacaine had better suppression of IgE responses than those receiving pDf11 +/- lipofectin or alum. Bestatin could greatly boost IgG2a responses. Immunomodulating effects of different adjuvants between protein and DNA vaccines were further elucidated. CpG was the best for both protein and DNA vaccines to profoundly suppress IgE responses, but alum, bestatin and lipofectin were useless for rDf11 to induce IgE inhibition. Neither did the combination of rDf11 and pDf11 have further IgE suppression. In conclusion, CpG is the unique adjuvant for the protein vaccine rDf11 to inhibit IgE responses. In contrast, the DNA vaccine pDf11 +/- CpG, bestatin, or bupivacaine induces profound suppression of IgE responses. (C) 2003 Elsevier Ltd. All rights reserved
机译：很难纯化用于免疫疗法的高分子量螨过敏原Der f11用于开发DNA疫苗pDf11。我们已经显示，用pDf11进行的疫苗接种可诱导以IgE反应抑制为特征的Th1反应。在本研究中，首先研究了不同佐剂对pDf11的作用。与接受pDf11 +/- lipofectin或明矾的小鼠相比，接受pDf11 +/- CpG，bestatin和布比卡因的小鼠对IgE反应的抑制作用更好。 Bestatin可以大大增强IgG2a反应。进一步阐明了蛋白质和DNA疫苗之间不同佐剂的免疫调节作用。 CpG是蛋白质和DNA疫苗中最能深刻抑制IgE反应的药物，但是明矾，贝斯汀和lipofectin对于rDf11不能诱导IgE抑制。 rDf11和pDf11的组合也没有进一步抑制IgE。总之，CpG是蛋白疫苗rDf11抑制IgE反应的独特佐剂。相比之下，DNA疫苗pDf11 +/- CpG，bestatin或布比卡因可诱导IgE反应的显着抑制。 （C）2003 Elsevier Ltd.保留所有权利

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