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首页> 外文期刊>Development >RPK2 is an essential receptor-like kinase that transmits the CLV3 signal in Arabidopsis.
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RPK2 is an essential receptor-like kinase that transmits the CLV3 signal in Arabidopsis.

机译：RPK2是一种必需的受体样激酶，可在拟南芥中传递CLV3信号。

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摘要

The shoot apical meristem (SAM) is the fundamental structure that is located at the growing tip and gives rise to all aerial parts of plant tissues and organs, such as leaves, stems and flowers. In Arabidopsis thaliana, the CLAVATA3 (CLV3) pathway regulates the stem cell pool in the SAM, in which a small peptide ligand derived from CLV3 is perceived by two major receptor complexes, CLV1 and CLV2-CORYNE (CRN)/SUPPRESSOR OF LLP1 2 (SOL2), to restrict WUSCHEL (WUS) expression. In this study, we used the functional, synthetic CLV3 peptide (MCLV3) to isolate CLV3-insensitive mutants and revealed that a receptor-like kinase, RECEPTOR-LIKE PROTEIN KINASE 2 (RPK2), also known as TOADSTOOL 2 (TOAD2), is another key regulator of meristem maintenance. Mutations in the RPK2 gene result in stem cell expansion and increased number of floral organs, as seen in the other clv mutants. These phenotypes are additive with both clv1 and clv2 mutations. Moreover, our biochemical analyses using Nicotiana benthamiana revealed that RPK2 forms homo-oligomers but does not associate with CLV1 or CLV2. These genetic and biochemical findings suggest that three major receptor complexes, RPK2 homomers, CLV1 homomers and CLV2-CRN/SOL2 heteromers, are likely to mediate three signalling pathways, mainly in parallel but with potential crosstalk, to regulate the SAM homeostasis.
机译：顶生分生组织（SAM）是位于生长尖端的基本结构，会引起植物组织和器官的所有空中部分，例如叶，茎和花。在拟南芥中，CLAVATA3（CLV3）途径调节着SAM中的干细胞池，其中两个主要受体复合物CLV1和CLV2-CORYNE（CRN）/ LLP1 2的抑制剂抑制了CLV3衍生的小肽配体（ SOL2），以限制WUSCHEL（WUS）表达。在这项研究中，我们使用功能性的合成CLV3肽（MCLV3）分离了对CLV3不敏感的突变体，并发现受体样激酶，受体样蛋白激酶2（RPK2）也称为TOADSTOOL 2（TOAD2）。分生组织维护的另一个关键调节器。 RPK2基因突变导致干细胞扩增，花器官数量增加，如其他clv突变体所示。这些表型是clv1和clv2突变的加和。此外，我们使用本氏烟草的生化分析显示，RPK2形成同源寡聚体，但不与CLV1或CLV2缔合。这些遗传和生化发现表明，三种主要的受体复合物RPK2同聚体，CLV1同聚体和CLV2-CRN / SOL2异聚体很可能介导三个信号通路，主要是平行的但具有潜在的串扰，以调节SAM的稳态。

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