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Identification and characterization of putative stem cells in the adult pig ovary

机译：成年猪卵巢中假定的干细胞的鉴定和表征

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Recently, the concept of 'neo-oogenesis' has received increasing attention, since it was shown that adult mammals have a renewable source of eggs. The purpose of this study was to elucidate the origin of theseeggsand to confirmwhether neo-oogenesis continuesthroughout life in the ovaries of the adultmammal. Adult female pigs were utilized to isolate, identify and characterize, including their proliferation and differentiation capabilities, putative stem cells (PSCs) from the ovary. PSCs were found to comprise a heterogeneous population based on c-kit expression and cell size, and also express stem and germ cell markers. Analysis of PSC molecular progression during establishment showed that these cells undergo cytoplasmic-to-nuclear translocation of Oct4 in a manner reminiscent of gonadal primordial germcells (PGCs). Hence, cells with the characteristics of early PGCs are present or are generated in the adult pig ovary. Furthermore, the in vitro establishment of porcine PSCs required the presence of ovarian cell-derived extracellular regulatory factors, which are also likely to direct stem cell niche interactions in vivo. In conclusion, the present work supports a crucial role for c-kit and kit ligand/stem cell factor in stimulating the growth, proliferation and nuclear reprogramming of porcine PSCs, and further suggests that porcine PSCs might be the culture equivalent of early PGCs.
机译：最近，“新卵子发生”的概念受到越来越多的关注，因为它表明成年哺乳动物具有可再生的卵子来源。这项研究的目的是阐明seeggs的起源，并确认新卵发生在整个成年哺乳动物卵巢中是否持续一生。用成年雌猪从卵巢中分离，鉴定和鉴定其推定的干细胞（PSC），包括其增殖和分化能力。发现PSC包含基于c-kit表达和细胞大小的异质群体，并且还表达干细胞和生殖细胞标记。建立过程中对PSC分子进程的分析表明，这些细胞以一种类似于性腺原始生殖细胞（PGCs）的方式经历了Oct4的胞质至核易位。因此，成年猪卵巢中存在或产生具有早期PGC特征的细胞。此外，猪PSC的体外建立需要卵巢细胞源性细胞外调节因子的存在，这也可能在体内指导干细胞生态位的相互作用。总之，本研究支持c-kit和kit配体/干细胞因子在刺激猪PSC的生长，增殖和核重编程中的关键作用，并进一步表明，猪PSC可能与早期PGC的培养相当。

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