首页> 外国专利> PLURIPOTENT HUMAN ADIPOSE ADULT STEM CELLS: ISOLATION, CHARACTERIZATION AND CLINICAL IMPLICATIONS

PLURIPOTENT HUMAN ADIPOSE ADULT STEM CELLS: ISOLATION, CHARACTERIZATION AND CLINICAL IMPLICATIONS

机译：多能人脂肪成人干细胞：分离，鉴定和临床意义

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摘要

Methods for the efficient isolation and use of pluripotent adipose-derived stem cells (PASCs) are provided. In certain embodiments the methods involve providing an adipose tissue sample from which the stromal vascular fraction is co-cultured with the adipocyte fraction. PASCs can be isolated with a high degree of purification without requiring an additional cell enrichment process (e.g. cell sorting). PASCs and their conditioned media can be used for tissue regeneration within hours of harvesting the adipose tissue, and without requiring cell expansion. PASCs can grow as floating individual cells, as clusters of cells, or attached to surface(s) of the culture vessel. PASCs do not produce teratomas in vivo, nor do they induce immunorejection upon transplantation, and they achieve a high efficiency in grafting. The cells and compositions can be used for cell therapy and to screen new drugs.

机译：提供了有效分离和使用多能脂肪干细胞（PASC）的方法。在某些实施方案中，该方法包括提供脂肪组织样品，从该脂肪组织样品中基质血管级分与脂肪细胞级分共培养。 PASC可以高度纯化，而无需其他细胞富集过程（例如细胞分选）。 PASC及其条件培养基可在收获脂肪组织后数小时内用于组织再生，而无需细胞扩增。 PASC可以作为漂浮的单个细胞，细胞簇或附着于培养容器表面的形式生长。 PASCs不会在体内产生畸胎瘤，也不会在移植后诱导免疫排斥，因此移植效率很高。该细胞和组合物可用于细胞治疗和筛选新药。

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公开/公告号EP3613846A1

专利类型
公开/公告日2020-02-26

原文格式PDF
申请/专利权人 THE REGENTS OF THE UNIVERSITY OF CALIFORNIA;
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申请/专利号EP20190202087
发明设计人 CHAZENBALK GREGORIO;
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申请日2014-05-22
分类号C12N5/0775;A61K35/12;A61P3/08;A61P25;A61P31;A61P35;A61P37;A61K35/28;C12N5/074;
国家 EP
入库时间 2022-08-21 11:38:23

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