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首页> 外文期刊>Development >The Xenopus LIM-homeodomain protein Xlim5 regulates the differential adhesion properties of early ectoderm cells.
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The Xenopus LIM-homeodomain protein Xlim5 regulates the differential adhesion properties of early ectoderm cells.

机译：爪蟾LIM-同源结构域蛋白Xlim5调节早期外胚层细胞的差异粘附特性。

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One of the earliest lineage restriction events in embryogenesis is the specification of the primary germ layers: ectoderm, mesoderm and endoderm. In Xenopus, germ layer specification occurs prior to gastrulation and requires the transcription factor VegT both for the cellautonomous specification of endoderm and the generation of mesoderm-inducing signals. In the absence of VegT, ectoderm is formed in all regions of the embryo. In this work, we show that VegT-depleted vegetal cells (prospective endoderm) behave like animal cells in sorting assays and ectopically express early markers of ectoderm. To gain insight into how ectoderm is specified, we looked for candidate ectoderm-specific genes that are ectopically expressed in VegT-depleted embryos, and examined the role of one of these, the LIM homeobox gene Xlim5, in ectoderm development. We show that overexpression of Xlim5 in prospective endoderm cells is sufficient to impair sorting of animal cells from vegetal cells but is not sufficient (at similar doses) to induce ectoderm-specific genes. In whole embryos, Xlim5 causes vegetal cells to segregate inappropriately to other germ layers and express late differentiation markers of that germ layer. Inhibition of Xlim5 function using an Engrailed repressor construct or a morpholino oligonucleotide causes loss of animal cell adhesion or delay in neural fold morphogenesis, respectively, without significantly affecting early ectoderm gene expression. Taken together, our results provide evidence that a primary role for Xlim5 is to specifically regulate differential cell adhesion behaviour of the ectoderm.
机译：胚胎发生中最早的谱系限制事件之一是主要胚芽层的规格：外胚层，中胚层和内胚层。在非洲爪蟾中，胚层规格发生在胃造气之前，并且对于内胚层的细胞自主规格和中胚层诱导信号的产生都需要转录因子VegT。在没有VegT的情况下，胚层在胚胎的所有区域形成。在这项工作中，我们表明，在分类测定中，VegT耗尽的植物细胞（预期的内胚层）的行为类似于动物细胞，并异位表达外胚层的早期标记。为了深入了解如何指定外胚层，我们寻找了在缺VegT的胚胎中异位表达的候选外胚层特异性基因，并研究了其中之一LIM同源盒基因Xlim5在外胚层发育中的作用。我们表明，预期动物内胚层细胞中Xlim5的过表达足以削弱植物细胞对动物细胞的分选，但不足以诱导外胚层特异性基因（以相似的剂量）。在整个胚胎中，Xlim5导致植物细胞不适当地分离至其他细菌层，并表达该细菌层的后期分化标记。使用Engrailed阻遏物构建物或吗啉代寡核苷酸抑制Xlim5功能分别导致动物细胞粘附丧失或神经折叠形态发生延迟，而不会显着影响早期外胚层基因表达。两者合计，我们的结果提供证据表明Xlim5的主要作用是特异性调节外胚层的差异细胞粘附行为。

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