...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Development >Muscle reconstitution by muscle satellite cell descendants with stem cell-like properties.
【24h】

Muscle reconstitution by muscle satellite cell descendants with stem cell-like properties.

机译：具有干细胞样特性的肌肉卫星细胞后代重建肌肉。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Recent studies have demonstrated that a distinct subpopulation with stem cell-like characteristics in myoblast culture is responsible for new muscle fiber formation after intramuscular transplantation. The identification and isolation of stem-like cells would have significant implications for successful myogenic cell transfer therapy in human muscle disorders. Using a clonal culture system for mouse muscle satellite cells, we have identified two cell types, designated 'round cells' and 'thick cells', in clones derived from single muscle satellite cells that have been taken from either slow or fast muscle. Clonal analysis of satellite cells revealed that the round cells are immediate descendants of quiescent satellite cells in adult muscle. In single-myofiber culture, round cells first formed colonies and then generated progeny, thick cells, that underwent both myogenic and osteogenic terminal differentiation under the appropriate culture conditions. Thick cells, but not round cells, responded to terminal differentiation-inducing signals. Round cells express Pax7, a specific marker of satellite cells, at high levels. Myogenic cell transfer experiments showed that round cells reconstitute myofibers more efficiently than thick cells. Furthermore, round cells restored dystrophin in myofibers of mdx nude mice, even when as few as 5000 cells were transferred into the gastrocnemius muscle. These results suggest that round cells are satellite-cell descendants with stem cell-like characteristics and represent a useful source of donor cells to improve muscle regeneration.
机译：最近的研究表明，成肌细胞培养物中具有干细胞样特征的独特亚群是肌内移植后新的肌纤维形成的原因。干细胞样细胞的鉴定和分离对成功地治疗人类肌肉疾病的成肌细胞具有重要意义。使用用于小鼠肌肉卫星细胞的克隆培养系统，我们从源自慢肌或快肌的单个肌肉卫星细胞衍生的克隆中鉴定出两种细胞类型，分别称为“圆形细胞”和“厚细胞”。卫星细胞的克隆分析表明，圆形细胞是成年肌肉中静止卫星细胞的直接后代。在单肌纤维培养中，圆形细胞首先形成菌落，然后生成后代厚细胞，在适当的培养条件下，它们经历了成肌和成骨的终末分化。厚细胞而非圆形细胞对终末分化诱导信号作出反应。圆形细胞高水平表达Pax7（一种卫星细胞的特异性标记物）。成肌细胞转移实验表明，圆形细胞比厚细胞更有效地重构肌纤维。此外，即使将多达5000个细胞转移到腓肠肌中，圆形细胞也可以恢复mdx裸鼠肌纤维中的肌营养不良蛋白。这些结果表明圆形细胞是具有干细胞样特征的卫星细胞后代，并代表了改善肌肉再生的有用的供体细胞来源。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号