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首页> 外文期刊>Development >Perivascular cells expressing annexin A5 define a novel mesenchymal stem cell-like population with the capacity to differentiate into multiple mesenchymal lineages.
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Perivascular cells expressing annexin A5 define a novel mesenchymal stem cell-like population with the capacity to differentiate into multiple mesenchymal lineages.

机译：表达膜联蛋白A5的血管周围细胞定义了一种新型的间充质干细胞样群体，具有分化为多种间充质谱系的能力。

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摘要

The annexin A5 gene (Anxa5) was recently found to be expressed in the developing and adult vascular system as well as the skeletal system. In this paper, the expression of an Anxa5-lacZ fusion gene was used to define the onset of expression in the vasculature and to characterize these Anxa5-lacZ-expressing vasculature-associated cells. After blastocyst implantation, Anxa5-lacZ-positive cells were first detected in extra-embryonic tissues and in angioblast progenitors forming the primary vascular plexus. Later, expression is highly restricted to perivascular cells in most blood vessels resembling pericytes or vascular smooth muscle cells. Viable Anxa5-lacZ+ perivascular cells were isolated from embryos as well as adult brain meninges by specific staining with fluorescent X-gal substrates and cell-sorting. These purified lacZ+ cells specifically express known markers of pericytes, but also markers characteristic for stem cell populations. In vitro and in vivo differentiation experiments show that this cell pool expresses early markers of chondrogenesis, is capable of forming a calcified matrix and differentiates into adipocytes. Hence, Anxa5 expression in perivascular cells from mouse defines a novel population of cells with a distinct developmental potential.
机译：最近发现，膜联蛋白A5基因（Anxa5）在发育中的和成年的血管系统以及骨骼系统中表达。在本文中，Anxa5-lacZ融合基因的表达被用来定义在脉管系统中表达的起点，并表征这些表达Anxa5-lacZ的脉管系统相关细胞。胚泡植入后，首先在胚外组织和形成初级血管丛的成血管祖细胞中检测到Anxa5-lacZ阳性细胞。后来，在大多数类似于周细胞或血管平滑肌细胞的血管中，表达高度受限于血管周细胞。通过用荧光X-gal底物进行特异性染色并进行细胞分选，从胚胎以及成人脑膜中分离出了可行的Anxa5-lacZ +血管周细胞。这些纯化的lacZ +细胞特异性表达周细胞的已知标记，但也表达干细胞群体的特征性标记。体外和体内分化实验表明，该细胞池表达软骨形成的早期标记，能够形成钙化基质并分化为脂肪细胞。因此，来自小鼠的血管周细胞中的Anxa5表达定义了具有独特发育潜力的新型细胞群。

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