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首页> 外文期刊>Development >Functional redundancy among Nanos proteins and a distinct role of Nanos2 during male germ cell development.
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Functional redundancy among Nanos proteins and a distinct role of Nanos2 during male germ cell development.

机译：Nanos蛋白之间的功能冗余以及Nanos2在雄性生殖细胞发育过程中的独特作用。

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摘要

The mouse Nanos proteins, Nanos2 and Nanos3, are required for germ cell development and share a highly conserved zinc-finger domain. The expression patterns of these factors during development, however, differ from each other. Nanos3 expression in the mouse embryo commences in the primordial germ cells (PGCs) just after their formation, and a loss of this protein results in the germ cell-less phenotype in both sexes. By contrast, Nanos2 expression begins only in male PGCs after their entry into the genital ridge and a loss of this protein results in a male germ cell deficiency, irrespective of the co-expression of Nanos3 in these cells. These results indicate that these two Nanos proteins have distinct functions, which depend on the time and place of their expression. To further elucidate this, we have generated transgenic mouse lines that express Nanos2 under the control of the Oct4DeltaPE promoter and examined Nanos2 function in a Nanos3-null genetic background. We find that ectopically produced Nanos2 protein rescues the Nanos3-null defects, because the germ cells fully develop in both sexes in the transgenic mice. This result indicates that Nanos2 can substitute for Nanos3 during early PGC development. By contrast, our current data show that Nanos3 does not rescue the defects in Nanos2-null mice. Our present findings thus indicate that there are redundant functions of the Nanos proteins in early PGC development, but that Nanos2 has a distinct function during male germ cell development in the mouse.
机译：小鼠Nanos蛋白Nanos2和Nanos3是生殖细胞发育所必需的，并具有高度保守的锌指结构域。但是，这些因素在发育过程中的表达方式互不相同。小鼠胚胎中的Nanos3表达在它们形成后即刻在原始生殖细胞（PGC）中开始，这种蛋白质的缺失导致男女两性无生殖细胞表型。相比之下，Nanos2的表达仅在雄性PGC进入生殖器后才开始，并且该蛋白的缺失会导致雄性生殖细胞缺乏，而与这些细胞中Nanos3的共表达无关。这些结果表明这两种纳米蛋白具有不同的功能，这取决于它们表达的时间和位置。为了进一步阐明这一点，我们已经生成了在Oct4DeltaPE启动子控制下表达Nanos2的转基因小鼠品系，并在Nanos3无基因背景中检查了Nanos2的功能。我们发现，异位产生的Nanos2蛋白可以挽救Nanos3-null缺陷，因为生殖细胞在转基因小鼠的两性中均能完全发育。该结果表明，在PGC的早期开发过程中，Nanos2可以替代Nanos3。相比之下，我们目前的数据表明Nanos3不能挽救Nanos2无小鼠的缺陷。因此，我们目前的发现表明，在PGC早期发育中Nanos蛋白具有多余的功能，但是Nanos2在小鼠雄性生殖细胞发育过程中具有独特的功能。

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