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首页> 外文期刊>Development Genes and Evolution >Differential expression of four protocadherin alpha and gamma clusters in the developing and adult zebrafish: DrPcdh2gamma but not DrPcdh1gamma is expressed in neuronal precursor cells, ependymal cells and non-neural epithelia
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Differential expression of four protocadherin alpha and gamma clusters in the developing and adult zebrafish: DrPcdh2gamma but not DrPcdh1gamma is expressed in neuronal precursor cells, ependymal cells and non-neural epithelia

机译：在发育中和成年斑马鱼中四个原钙粘蛋白α和γ簇的差异表达：DrPcdh2gamma但不是DrPcdh1gamma在神经元前体细胞，室间隔细胞和非神经上皮细胞中表达

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摘要

Protocadherins are cadherin-like molecules with adhesive and signaling functions, in particular, during neuronal development. Large protocadherin (Pcdh) gene clusters are present in the genome of vertebrates. In the zebrafish, two Pcdh clusters are found on chromosomes 10 (DrPcdh1) and 14 (DrPcdh2), each divided into subclusters of DrPcdhalpha and DrPcdhgamma family genes. In total, about 100 different DrPcdh molecules are predicted. We have analyzed the expression of the four DrPcdh subclusters and find that DrPcdh transcripts are upregulated in the developing zebrafish nervous system. In the adult fish brain, all four DrPcdh clusters are expressed in differentiated neurons, in particular, in the thalamic nuclei, tectum, and cerebellum. We show that expression patterns grossly overlap for each cluster but with regional differences and variations in strength of expression. Strikingly, the DrPcdh2gamma cluster, distinct from the three other clusters, is also expressed in neuronal precursor cells and ependymal cells of the embryonic and adult nervous system, as well as in specific non-neuronal epithelia. Antibodies to a conserved motif in the constant region of DrPcdh2gamma stain fiber tracts and neuropil of the zebrafish brain and cell-cell junctions in epithelia. Our results indicate that multiple DrPcdhs of the different clusters are expressed in differentiated zebrafish neurons, suggesting evolutionarily conserved functions of protocadherin clusters in cell adhesion and signaling. In addition, DrPcdh2gamma may exert more specific roles in neuronal precursor and non-neural epithelial cells, which have not yet been described for mammalian Pcdhgamma. Thus, our findings in zebrafish open new perspectives to examine these functions in other vertebrate model organisms.
机译：原始钙粘着蛋白是具有粘附和信号传导功能的钙粘着蛋白样分子，特别是在神经元发育过程中。脊椎动物基因组中存在大型原钙粘蛋白（Pcdh）基因簇。在斑马鱼中，在10号染色体（DrPcdh1）和14号染色体（DrPcdh2）上发现了两个Pcdh簇，每个簇分为DrPcdhalpha和DrPcdhgamma家族基因的子簇。总共预测出约100种不同的DrPcdh分子。我们分析了四个DrPcdh子簇的表达，发现在发育中的斑马鱼神经系统中DrPcdh转录本上调。在成年鱼脑中，所有四个DrPcdh簇均在分化的神经元中表达，尤其是在丘脑核，顶盖和小脑中。我们显示每个群集的表达模式完全重叠，但表达强度存在区域差异和变化。令人惊讶的是，不同于其他三个簇的DrPcdh2gamma簇也表达于胚胎和成年神经系统的神经元前体细胞和室间隔膜细胞，以及特定的非神经上皮细胞中。 DrPcdh2gamma染色区的恒定区域中的保守基序的抗体，以及斑马鱼大脑和上皮细胞连接处的神经纤维。我们的结果表明，在分化的斑马鱼神经元中表达了不同簇的多个DrPcdhs，这表明原钙粘蛋白簇在细胞粘附和信号传导中的进化保守功能。此外，DrPcdh2gamma可能在神经元前体细胞和非神经上皮细胞中发挥更具体的作用，而哺乳动物Pcdhgamma尚未被描述。因此，我们在斑马鱼中的发现为检查其他脊椎动物模型生物中的这些功能开辟了新的前景。

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