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首页> 外文期刊>Chromosome research: An international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology >Molecular cytogenetic characterization and seed storage protein analysis of 1A/1D translocation lines of durum wheat.
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Molecular cytogenetic characterization and seed storage protein analysis of 1A/1D translocation lines of durum wheat.

机译：硬粒小麦1A / 1D易位系的分子细胞遗传学表征和种子贮藏蛋白分析。

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Two durum wheat [Triticum turgidum L. ssp. durum (Desf.) Husn.] lines carrying the high-molecular-weight (HMW) glutenin subunits (GS) 1Dx5 + 1Dy10 encoded by Glu-D1d, L252 and S99B34, were characterized using fluorescent genomic in-situ hybridization (FGISH) and microsatellite markers. These two durum lines were derived from the crosses in which the common wheat (T. aestivum L.) 'Len' and durum wheat 'Langdon' (LDN) and 'Renville' were involved. FGISH patterns of the mitotic chromosomes indicated that these two durum lines have one pair of 1AS.1AL-1DL translocated chromosomes in which the terminal region of 1AL was replaced by a homoeologous segment of 1DL. The 1DL segment spans approximately 31% of the long arm of the translocated chromosome. Microsatellite marker analysis confirmed the 1AS.1AL-1DL translocation and determined the translocation breakpoint to be distal to Xgwm357 on 1AL. Seed storage proteins (GS and gliadins) were analysed in these two 1AS.1AL-1DL translocation lines and three sib lines (L092, S99B19 and S99B33) using SDS-PAGE and A-PAGE. The SDS-PAGE and A-PAGE profiles demonstrated that the two low yielding lines (L252 and S99B19) had the low-molecular-weight (LMW) -1 GS encoded by Glu-A3k and Glu-B3s and 1B-encoded gliadins from LDN, and the other three lines (L092, S99B33 and S99B34) with higher yield had LMW-2 GS and 1B-encoded gliadins from Renville, suggesting that undesirable genetic components from LDN might limit substantial improvement of yield. Thus, the translocation lines with 1Dx5 + 1Dy10 and LMW-2, which are associated with good bread-making and pasta qualities, respectively, in a good genetic background will be useful for developing durum cultivars with dual-purpose end-use. Results from this study demonstrate that the D-genome could play an important role in the genetic improvement of durum wheat and evolution of the A- and B-genomes in tetraploid wheat.
机译：两个硬质小麦[Triticum turgidum L. ssp。用荧光基因组原位杂交（FGISH）和Glu-D1d，L252和S99B34编码的带有高分子量（HMW）谷蛋白亚基（GS）1Dx5 + 1Dy10的Durum（Desf。Husn。）品系微卫星标记。这两个硬质小麦品系均来自杂交，其中涉及普通小麦（T. aestivum L。）“ Len”，硬质小麦“ Langdon”（LDN）和“ Renville”。有丝分裂染色体的FGISH图谱表明这两个硬粒细胞系具有一对1AS.1AL-1DL易位染色体，其中1AL的末端区域被1DL的同源片段代替。 1DL片段大约占易位染色体长臂的31％。微卫星标记分析证实了1AS.1AL-1DL易位，并确定了易位断点位于1AL上Xgwm357的远端。使用SDS-PAGE和A-PAGE在这两个1AS.1AL-1DL易位系和三个sib系（L092，S99B19和S99B33）中分析了种子贮藏蛋白（GS和麦醇溶蛋白）。 SDS-PAGE和A-PAGE图谱表明，两条低产系（L252和S99B19）具有由LDN的Glu-A3k和Glu-B3s编码的低分子量（LMW）-1 GS和1B编码的麦醇溶蛋白，以及其他三个具有较高产量的品系（L092，S99B33和S99B34）具有来自Renville的LMW-2 GS和1B编码的麦醇溶蛋白，这表明LDN的不良遗传成分可能会限制产量的大幅提高。因此，在良好的遗传背景下，分别具有良好的制面包和面食品质的1Dx5 + 1Dy10和LMW-2易位系将有助于开发具有双重用途的硬粒小麦品种。这项研究的结果表明，D基因组可以在硬粒小麦的遗传改良以及四倍体小麦的A基因和B基因组的进化中发挥重要作用。

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