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首页> 外文期刊>Turkish Journal of Agriculture & Forestry >Chloroplast DNA variation and pollen contamination in a Pinus brutia Ten. clonal seed orchard: implication for progeny performance in plantations
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Chloroplast DNA variation and pollen contamination in a Pinus brutia Ten. clonal seed orchard: implication for progeny performance in plantations

机译：松树十中的叶绿体DNA变化和花粉污染。克隆种子园：对人工林后代表现的影响

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摘要

Pollen contamination is one of the important factors affecting the yield, adaptability, and genetic quality of the seed produced from seed orchards in forest tree breeding programs. Incoming pollen from the forests surrounding the seed orchard is a major concern in tree breeding because it contributes to losses in the expected genetic gains from seed orchard crops. The genetic variation and the level of pollen contamination in a 16-year-old Pinus brutia Ten. first-generation clonal seed orchard was studied using chloroplast microsatellite markers (cpSSRs). In total, 23 alleles and 36 unique allelic combinations (haplotypes) were detected based on the 6 cpSSR loci analyzed. The haplotypic diversity of the clones in the seed orchard was found to be 0.849. Out of 300 embryos analyzed, 87 were not compatible with any male parent within the seed orchard. Thus, 29% of the embryos were sired by pollen sources outside the orchard (i.e. apparent contamination). Microsatellite-based analysis revealed that the estimated contamination rate was 39.3%. Background pollination at this level will cause losses of 20% in the expected genetic gains. Our findings are valuable for the assessment of the intended seed orchard function, i.e. provision of genetically improved seed. It may be worthwhile to use pollen management strategies like strobilus stimulation, controlled pollination, and supplemental mass pollination to decrease pollen contamination and increase the genetic quality of the seeds produced.
机译：花粉污染是影响林木育种计划中从种子园生产的种子的产量，适应性和遗传质量的重要因素之一。从种子园周围的森林中传入的花粉是树木育种中的一个主要问题，因为它导致种子园作物预期的遗传增益损失。 16岁的Pinus brutia 10的遗传变异和花粉污染水平。使用叶绿体微卫星标记（cpSSRs）研究了第一代克隆种子园。根据所分析的6个cpSSR基因座，总共检测到23个等位基因和36个独特的等位基因组合（单倍型）。发现种子园中克隆的单倍型多样性为0.849。在分析的300个胚胎中，有87个与种子园中的任何雄性亲本都不相容。因此，有29％的胚胎被果园外的花粉来源诱捕（即明显的污染）。基于微卫星的分析表明，估计污染率为39.3％。在这个水平上的背景授粉将导致预期遗传增益损失20％。我们的发现对于评估预期的种子园功能，即提供经遗传改良的种子具有重要价值。可能有必要使用诸如花粉刺激，控制传粉和补充传粉的花粉管理策略来减少花粉污染并提高所产生种子的遗传质量。

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