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Transcriptomic analysis of tomato lines reveals putative stress-specific biomarkers

机译:番茄品系的转录组学分析揭示了假定的胁迫特异性生物标记

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摘要

Different abiotic stresses recruit dedicated signaling and regulatory genes in plants. Genome-wide stress-specific biomarkers were investigated in tomatoes. Three major abiotic stresses were compared: drought, heat, and salinity. For each stress type,2 different tomato lines were included: susceptible and tolerant. Gene expression was examined by hybridizing to an available tomato microarray. Several stress responsive genes were upregulated in tolerant as well as in susceptible lines for each stress. Comparative analysis of gene expression in response to stress (drought, heat, or salinity) resolved a number of common biomarkers, while other groups of putative biomarkers were associated with each abiotic stress. MYB transcription factors, SAUR family proteins, and NAしaomain proteins were among the highly upregulated genes under drought, while both proteinase inhibitors and heat shock proteins were prominent in the heat-tolerant line. For salinity stress, the expression of phosphate starvation-induced proteins was observed. Putative abiotic stress biomarkers can be utilized in breeding programs to improve the selection process and to aia m gene stacking.
机译:不同的非生物胁迫在植物中募集了专用的信号传导和调节基因。在番茄中研究了全基因组胁迫特异性生物标记。比较了三种主要的非生物胁迫:干旱,高温和盐度。对于每种胁迫类型,包括2种不同的番茄品系:易感和耐性。通过与可用的番茄微阵列杂交检查基因表达。每种胁迫在耐性和易感系中都上调了几种胁迫响应基因。基因表达对胁迫(干旱,热或盐度)的响应的比较分析解决了许多常见的生物标记,而其他假定的生物标记则与每种非生物胁迫相关。在干旱条件下,MYB转录因子,SAUR家族蛋白和NAしaomain蛋白是高度上调的基因,而蛋白酶抑制剂和热激蛋白在耐热系中均很突出。对于盐分胁迫,观察到磷酸饥饿诱导的蛋白的表达。推定的非生物胁迫生物标志物可用于育种程序,以改善选择过程并改善基因堆叠。

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