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首页> 外文期刊>Turkish journal of biology >Evaluation of the genetic relationship between Fritillaria species from Turkey's flora using fluorescent-based AFLP
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Evaluation of the genetic relationship between Fritillaria species from Turkey's flora using fluorescent-based AFLP

机译：使用基于荧光的AFLP评估土耳其植物群中贝母物种之间的遗传关系

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In this study, the genetic relationships among 12 Fritillaria taxa collected from different regions of Turkey were investigated using the amplified fragment length polymorphism (AFLP) technique. Seven primer pair combinations were used for the analysis. The neighbor-joining and principal coordinate analysis methods were implemented to identify the taxonomy of the species. The analyses generated congruent results with each other and with the previous studies. The neighbor-joining analysis clustered the species into 3 groups, while the principal coordinates analysis did not support separation of the third group. The subgenera Fritillaria and Petilium diverged from each other with strong support in all analyses. The neighbor-joining tree further divided the members of subgenus Fritillaria into 2 subgroups. Statistical analysis of the data set using NTSYS 2.1 showed a very good fit between the data matrix and the cophenetic matrix for the AFLP data with an r-value of 0.91. Generating a high polymorphic band profile, this analysis presented the feasibility of using AFLP for a genetic relationship analysis of Fritillaria. This primary report on AFLP analysis of Fritillaria species could facilitate achieving a deeper understanding of genetic relationships between the species.
机译：在这项研究中，使用扩增片段长度多态性（AFLP）技术研究了从土耳其不同地区收集的12个贝母类群之间的遗传关系。七个引物对组合用于分析。实施了邻域联接和主坐标分析方法以识别物种的分类。这些分析相互之间以及与先前的研究均产生了一致的结果。邻居加入分析将物种分为3组，而主坐标分析不支持第三组的分离。在所有分析中，贝母亚科和贝母属彼此分离。相邻的树进一步将贝母亚属的成员分为两个亚组。使用NTSYS 2.1对数据集进行统计分析表明，对于AFLP数据，数据矩阵与同色矩阵之间的拟合度很好，r值为0.91。产生高的多态性带谱，此分析表明了使用AFLP进行贝母遗传关系分析的可行性。关于贝母物种的AFLP分析的主要报告可以促进对物种之间遗传关系的更深入了解。

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