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首页> 外文期刊>Turkish journal of biology >Identification of an AFLP marker linked with yellow rust resistance in wheat (Triticum aestivum L.)
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Identification of an AFLP marker linked with yellow rust resistance in wheat (Triticum aestivum L.)

机译：与小麦抗黄锈性相关的AFLP标记的鉴定

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摘要

An important application of molecular markers in plant systems involves improvement in the efficiency of conventional plant breeding by carrying out indirect selection through molecular markers linked to the traits of interest. AFLP analysis was used to identify molecular markers associated with yellow rust resistance in wheat (Triticum aestivum L.) in this study. DNA isolated from the selected yellow rust tolerant and susceptible F_2 individuals derived from a cross between Izgi2001 (resistant) and ES14 (susceptible) at seedling and adult stage was used for bulked segregant analysis combined with AFLP. From the screening of 34 PstI/MseI AFLP primer combinations, the AFLP marker P-GAC/M-ACG (133 bp) was identified and presented in the resistant parent and resistant F_2 individuals but not in the susceptible ones. Future research will obtain more adjacent sequences associated with the polymorphic M-ACG/P-GAC_(133bp) marker by the PCR walking method to design SCAR markers for wheat breeding programs.
机译：分子标记物在植物系统中的重要应用涉及通过与相关性状相关的分子标记物进行间接选择，从而提高常规植物育种的效率。在这项研究中，AFLP分析被用于鉴定与小麦（Triticum aestivum L.）的抗黄锈性相关的分子标记。从苗期和成年期从Izgi2001（抗性）和ES14（易感）之间的杂交中选出的耐黄锈和易感F_2个体中分离的DNA用于与AFLP结合的大量分离子分析。通过筛选34种PstI / MseI AFLP引物组合，鉴定了AFLP标记P-GAC / M-ACG（133 bp），并在抗性亲本和抗性F_2个体中显示，但在易感个体中未显示。未来的研究将通过PCR步移方法获得更多与多态性M-ACG / P-GAC_（133bp）标记相关的相邻序列，以设计用于小麦育种程序的SCAR标记。

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