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首页> 外文期刊>Chromosome research: An international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology >Genetic variation exists for telomeric array organization within and among the genomes of normal, immortalized, and transformed chicken systems.
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Genetic variation exists for telomeric array organization within and among the genomes of normal, immortalized, and transformed chicken systems.

机译：在正常，永生化和转化的鸡系统的基因组之内和之中，存在端粒阵列组织的遗传变异。

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This study investigated telomeric array organization of diverse chicken genotypes utilizing in vivo and in vitro cells having phenotypes with different proliferation potencies. Our experimental objective was to characterize the extent and nature of array variation present to explore the hypothesis that mega-telomeres are a universal and fixed feature of chicken genotypes. Four different genotypes were studied including normal (UCD 001, USDA-ADOL Line 0), immortalized (DF-1), and transformed (DT40) cells. Both cytogenetic and molecular approaches were utilized to develop an integrated view of telomeric array organization. It was determined that significant variation exists within and among chicken genotypes for chromosome-specific telomeric array organization and total genomic-telomeric sequence content. Although there was variation for mega-telomere number and distribution, two mega-telomere loci were in common among chicken genetic lines (GGA 9 and GGA W). The DF-1 cell line was discovered to maintain a complex derivative karyotype involving chromosome fusions in the homozygous and heterozygous condition. Also, the DF-1 cell line was found to contain the greatest amount of telomeric sequence per genome (17%) as compared to UCD 001 (5%) and DT40 (1.2%). The chicken is an excellent model for studying unique and universal features of vertebrate telomere biology, and characterization of the telomere length variation among genotypes will be useful in the exploration of mechanisms controlling telomere length maintenance in different cell types having unique phenotypes.
机译：这项研究利用具有不同增殖潜能表型的体内和体外细胞研究了多种鸡基因型的端粒阵列组织。我们的实验目标是表征目前存在的阵列变异的程度和性质，以探讨巨型端粒是鸡基因型的普遍且固定的特征这一假设。研究了四种不同的基因型，包括正常（UCD 001，USDA-ADOL系0），永生化（DF-1）和转化（DT40）细胞。利用细胞遗传学和分子学方法来发展端粒阵列组织的综合观点。已经确定，针对染色体特异性端粒阵列组织和总基因组端粒序列含量，鸡基因型内和之间存在显着差异。尽管巨型端粒的数量和分布存在差异，但是在鸡遗传系中有两个巨型端粒位点是共同的（GGA 9和GGA W）。发现DF-1细胞系在纯合和杂合条件下维持复杂的染色体核型融合，涉及染色体融合。此外，发现与UCD 001（5％）和DT40（1.2％）相比，DF-1细胞系每个基因组含有最多的端粒序列（17％）。鸡是研究脊椎动物端粒生物学独特和普遍特征的优秀模型，基因型之间端粒长度变异的表征将有助于探索控制具有独特表型的不同细胞类型中端粒长度维持的机制。

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