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首页> 外文期刊>Turkish Journal of Botany >The assessment of genetic diversity of Castanea species by RAPD, AFLP, ISSR, and SSR markers
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The assessment of genetic diversity of Castanea species by RAPD, AFLP, ISSR, and SSR markers

机译：利用RAPD，AFLP，ISSR和SSR标记评估板栗种的遗传多样性

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Castanea Mill. (chestnut) is a multipurpose deciduous tree and member of the family Fagaceae, widely distributed throughout North America, Europe, and Asia. The evaluation of the genetic diversity of chestnut species is crucial for the effective conservation of this economically and ecologically valuable tree. In this study, we applied 4 DNA markers to detect the genetic variability among and within Castanea species and to compare the effectiveness of each system in estimating genetic variation. We amplified 106 random amplified polymorphic DNA (RAPD), 228 amplified fragment length polymorphism (AFLP), 42 intersimple sequence repeat (ISSR), and 36 simple sequence repeat (SSR) polymorphic markers using 12, 5, 4, and 5 primer combinations, respectively. The findings on the effective multiplex ratio, polymorphism information content, and marker index revealed that AFLP was the most effective molecular marker system used in this study. Each marker system classified the species under investigation into clear but incompletely separated clusters, although partial agreement was achieved with respect to species relationships when the RAPD method was employed. The comparison of the correlation coefficient of RAPD marker data and the other markers showed a higher correlation [(r = 0.69, P < 0.01), (r = 0.77, P < 0.01), and (r = 0.47, P <0.01) with AFLP, ISSR, and SSR, respectively]. When variance was partitioned among and within groups, AFLP (94.62%) showed greater variation within the groups and reverse RAPD (67.87%) yielded greater variation among the groups. Overall, the results indicate that the AFLP represents an efficient molecular marker system for the assessment of chestnut genetic diversity and, hence, the development of effective conservation strategies to preserve this valuable tree species
机译：板栗磨。 （栗子）是一棵多用途的落叶乔木，隶属于菊科（Fagaceae），广泛分布于北美，欧洲和亚洲。板栗树种遗传多样性的评估对于有效保护这种具有经济和生态价值的树木至关重要。在这项研究中，我们应用了4个DNA标记来检测栗树种之间和内部的遗传变异，并比较每个系统在评估遗传变异中的有效性。我们使用12、5、4和5个引物组合扩增了106个随机扩增多态性DNA（RAPD），228个扩增片段长度多态性（AFLP），42个单序列重复序列（ISSR）和36个单序列重复序列（SSR）多态性标记，分别。有效多路复用率，多态性信息含量和标记指数的发现表明，AFLP是本研究中使用的最有效的分子标记系统。尽管使用RAPD方法在物种关系方面达成了部分共识，但每个标记系统都将要调查的物种分为清晰但不完全分开的簇。 RAPD标记数据与其他标记的相关系数的比较显示出更高的相关性[（r = 0.69，P <0.01），（r = 0.77，P <0.01），（r = 0.47，P <0.01）。 AFLP，ISSR和SSR]。当在组间和组内划分方差时，AFLP（94.62％）显示组内较大的变异，而反向RAPD（67.87％）产生组间较大的变异。总体而言，结果表明，AFLP代表了一种有效的分子标记系统，可用于评估栗子的遗传多样性，因此，可以开发出有效的保护策略来保护这种宝贵的树种

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