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首页> 外文期刊>Developmental cell >Differential Roles of WAVE1 and WAVE2 in Dorsal and Peripheral Ruffle Formation for Fibroblast Cell Migration
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Differential Roles of WAVE1 and WAVE2 in Dorsal and Peripheral Ruffle Formation for Fibroblast Cell Migration

机译：WAVE1和WAVE2在成纤维细胞迁移的背侧和周边褶皱形成中的差异作用

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摘要

Cell migration is driven by actin polymerization at the leading edge of lamellipodia, where WASP family verprolin-homologous proteins (WAVEs) activite Arp2/3 complex. When fibroblasts are stimulated with PDGF, formation of peripheral ruffles precedes that of dorsal ruffles in lamellipodia. Here, we show that WAVE2 deficiency impairs peripheral ruffle formation and WAVE1 deficiency impairs dorsal ruffle formation. During directed cell migration in the absence of extracellular matrix (ECM), cells migrate with peripheral ruffles at the leading edge and WAVE2, but not WAVE1, is essential. In contrast, both WAVE1 and WAVE2 are essential for invading migration into ACM, suggesting that the leading edge in ACM has characteristics of both ruffles. WAVE1 is colocalized when ACM-degrading enzyme MMP-2 in dorsal urfles, and WAVE1-, but not WAVE2-, dependent migration requires MMP activity. Thus, WAVE2 is essential for leading edge extension for directed migration in general and WAVE1 is essential in MMP-dependent migration in ACM.
机译：细胞迁移是由lamellipodia的前沿的肌动蛋白聚合驱动的，在那里WASP家族verprolin-同源蛋白（WAVEs）激活Arp2 / 3复合物。当用PDGF刺激成纤维细胞时，在片状脂膜病中外周褶皱的形成先于背褶皱的形成。在这里，我们表明WAVE2缺乏症损害周围的皱纹形成，而WAVE1缺乏症损害背侧的皱纹形成。在没有细胞外基质（ECM）的情况下进行定向细胞迁移期间，细胞在前缘带有外围褶皱迁移，因此WAVE2（而不是WAVE1）是必不可少的。相比之下，WAVE1和WAVE2对于入侵迁移到ACM都是必不可少的，这表明ACM的前沿具有两种褶皱的特征。当背侧ACM降解酶MMP-2和WAVE1而不是WAVE2-依赖迁移需要MMP活性时，WAVE1是共定位的。因此，一般来说，WAVE2对于前沿扩展对于定向迁移必不可少，而WAVE1在ACM中依赖MMP的迁移中必不可少。

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