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A regulatory framework for shoot stem cell control integrating metabolic, transcriptional, and phytohormone signals

机译:芽干细胞控制的整合代谢,转录和植物激素信号的调控框架

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摘要

Plants continuously maintain pluripotent stem cells embedded in specialized tissues called meristems, which drive long-term growth and organogenesis. Stem cell fate in the shoot apical meristem (SAM) is controlled by the homeodomain transcription factor WUSCHEL (WUS) expressed in the niche adjacent to the stem cells. Here, we demonstrate that the bHLH transcription factor HECATE1 (HEC1) is a target of WUS and that it contributes to SAM function by promoting stem cell proliferation, while antagonizing niche cell activity. HEC1 represses the stem cell regulators WUS and CLAVATA3 (CLV3) and, like WUS, controls genes with functions in metabolism and hormone signaling. Among the targets shared by HEC1 and WUS are phytohormone response regulators, which we show to act as mobile signals in a universal feedback system. Thus, our work sheds light on the mechanisms guiding meristem function and suggests that the underlying regulatory system is far more complex than previously anticipated.
机译:植物会持续保持多能干细胞嵌入到称为分生组织的特殊组织中,从而驱动长期生长和器官发生。茎尖分生组织(SAM)中的干细胞命运受与干细胞相邻的小生境中表达的同源域转录因子WUSCHEL(WUS)的控制。在这里,我们证明bHLH转录因子HECATE1(HEC1)是WUS的靶标,并且它通过促进干细胞增殖而对抗小生境细胞活性,从而有助于SAM功能。 HEC1抑制干细胞调节剂WUS和CLAVATA3(CLV3),并且像WUS一样,控制具有代谢和激素信号传导功能的基因。植物激素反应调节剂是HEC1和WUS共有的靶标,我们证明了它们在通用反馈系统中可作为移动信号发挥作用。因此,我们的工作揭示了指导分生组织功能的机制,并表明基础的监管体系比以前预期的要复杂得多。

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