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首页> 外文期刊>Domestic Animal Endocrinology >Possible role of IGF2 receptors in regulating selection of 2 dominant follicles in cattle selected for twin ovulations and births
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Possible role of IGF2 receptors in regulating selection of 2 dominant follicles in cattle selected for twin ovulations and births

机译：IGF2受体在调节双胎排卵和分娩的牛中2个主要卵泡选择中的可能作用

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Abundance of IGF-2 receptor (IGF2R), FSH receptor (FSHR), and LH receptor (LHCGR) mRNA in granulosa cells (GCs) or theca cells (TCs) or both cells as well as estradiol (E-2), progesterone (P-4), and androstenedione concentrations in follicular fluid were compared in cows genetically selected (Twinner) or not selected (control) for multiple ovulations and twin births. Cows were slaughtered at day 3 to 4 (day 3) and day 5 to 6 (day 5) of an estrous cycle, and ovaries, follicular fluid, GCs, and TCs were collected. The two largest (Fl and F2) E-2-active (EA) and E-2-inactive (EI) follicles were selected according to their E-2-to-P-4 ratio and diameter. Androstenedione levels in EA Fl and F2 follicles were 5-fold greater (P < 0.05) in Twinner cows than in control cows on day 3 but did not differ on day 5. Twinner cows also had greater (P < 0.05) E2 and P4 concentrations, whereas steroid levels in El follicles did not differ (P > 0.10) between genotypes. In EA F2 follicles, IGF2R levels in GCs were greater (P < 0.05) in control cows than in Twinner cows on day 3 and day 5, whereas IGF2R mRNA in TCs did not differ (P > 0.10). On day 3, FSHR mRNA levels were greater (P < 0.05) in GCs of EA Fl and El F2 follicles of control cows than of Twinner cows. LH receptor mRNA expression was less in GCs and greater in TCs of EA F2 follicles in control cows than in Twinner cows (P < 0.05). We hypothesize that reduced GC IGF2R expression in F2 follicles of Twinner cows may play a role in the development of 2 or more dominant follicles
机译：颗粒细胞（GCs）或卵泡膜细胞（TCs）或两种细胞以及雌二醇（E-2），孕酮（）中的IGF-2受体（IGF2R），FSH受体（FSHR）和LH受体（LHCGR）mRNA丰富P-4）和卵母细胞中雄烯二酮的浓度在经过多次排卵和双胞胎的遗传选择（双胞胎）或未选择（对照）的母牛中进行了比较。在发情周期的第3至4天（第3天）和第5至6天（第5天）宰杀母牛，并收集卵巢，卵泡液，GC和TC。根据它们的E-2-与P-4的比率和直径选择两个最大的（F1和F2）E-2-活性（EA）和E-2-非活性（EI）卵泡。在第3天，双胞胎牛的EA F1和F2卵泡中的雄烯二酮水平比对照牛高5倍（P <0.05），但在第5天没有差异。双胞胎牛的E2和P4浓度也更高（P <0.05） ，而基因型之间El卵泡中的类固醇水平没有差异（P> 0.10）。在EA F2卵泡中，在第3天和第5天，对照母牛的GC中IGF2R水平高于Twinner母牛（P <0.05），而TC中的IGF2R mRNA没有差异（P> 0.10）。在第3天，对照母牛的EA F1和E1 F2卵泡的GC中的FSHR mRNA水平更高（P ＜0.05）。与Twinner奶牛相比，对照奶牛的LH受体mRNA表达在GC中较少，在EA ​​F2卵泡的TC中则更高（P <0.05）。我们假设Twinner奶牛F2卵泡中GC IGF2R表达的降低可能在2个或更多优势卵泡的发育中起作用

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