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首页> 外文期刊>DNA Sequence: The Journal of DNA Sequencing and Mapping >Molecular cloning and sequence analysis of a novel chalcone synthase cDNA from Ginkgo biloba
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Molecular cloning and sequence analysis of a novel chalcone synthase cDNA from Ginkgo biloba

机译：银杏新查尔酮合酶cDNA的分子克隆与序列分析

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摘要

A chalcone synthase (CHS) gene was cloned from Ginkgo biloba for the first time and it was also the first cloned gene involved in flavonoids metabolic pathway in G. biloba. The full-length cDNA of G. biloba CHS (designated as Gbchs) was 1608 bp with poly(A) tailing and it contained a 1173 bp open reading frame (ORF) encoding a 391 amino acid protein. Gbchs was found to have extensive homology with those of other plant chs genes via multiple alignments. The active sites of the CoA binding, coumaroyl pocket and cyclization pocket in CHS protein of Medicago sativa were also found in GbCHS. Molecular modeling of GbCHS indicated that the three-dimensional structure of GbCHS strongly resembled that of M. sativa (MsCHS2), implying GbCHS may have similar functions with MsCHS2. Phylogenetic tree analysis revealed that GbCHS had closer relationship with CHSs from gymnosperm plants than from other plants. Gbchs is a useful tool to study the regulation of flavonoids metabolism in G. biloba.
机译：首次从银杏中克隆了查尔酮合酶（CHS）基因，这也是第一个与银杏黄酮代谢途径有关的克隆基因。 G. biloba CHS（命名为Gbchs）的全长cDNA为1608 bp，带有poly（A）尾部，并且包含1173 bp的开放阅读框（ORF），编码391个氨基酸。通过多重比对，发现Gbchs与其他植物chs基因具有广泛的同源性。在GbCHS中也发现了紫花苜蓿CHS蛋白中CoA结合，香豆酰基袋和环化袋的活性位点。 GbCHS的分子模型表明，GbCHS的三维结构与苜蓿（MsCHS2）的三维结构非常相似，这表明GbCHS可能与MsCHS2具有相似的功能。系统进化树分析显示，GbCHS与裸子植物中的CHS相比，与其他植物中的CHS具有更紧密的关系。 Gbchs是研究银杏叶黄酮代谢调控的有用工具。

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