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首页> 外文会议>International conference on informatics and management science;IMS 2012 >Molecular Cloning and Sequences Analysis of Chalcone Synthase Gene from Fagopyrum Tataricum
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Molecular Cloning and Sequences Analysis of Chalcone Synthase Gene from Fagopyrum Tataricum

机译：苦荞麦查尔酮合酶基因的分子克隆与序列分析

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One of the most important key enzymes Chalcone Synthase (CHS) gene was cloned from Fagopyrum tataricum. Based on cDNA sequence conserved domain of CHS, a pair of primers were designed and used to amplify a fragment of FtCHS gene (GenBank accession: EU715255) using RT-PCR and RACE technique. A 1,463 bp full-length cDNA sequence was obtained. Analysis of CHS cDNA indicated that it encoded a peptide containing 393 amino acids. The sequence identity to palm leaf rhubarb (Rheumpahlmatum L.) species was all about 83 %. It is predicted that secondary structure of FtCHS contains α-helix (41.73 %), layers (16.28 %) and random coils (41.98 %); further research on three-dimensional modeling of FtCHS contains 11 α-helixes and 15 β-layers, which showed that protein was able to fold to a typical three-dimensional structure of CHS protein highly similar to that of MsCHS 2. The predicted tertiary structure demonstrated that FtCHS has combination sites.
机译：最重要的关键酶查耳酮合酶（CHS）基因之一是从苦荞麦（Fagopyrum tataricum）克隆的。基于CHS的cDNA序列保守域，设计了一对引物，并利用RT-PCR和RACE技术扩增了FtCHS基因的片段（GenBank登录号：EU715255）。获得了1,463 bp的全长cDNA序列。 CHS cDNA的分析表明，它编码包含393个氨基酸的肽。与棕榈叶大黄（Rheumpahlmatum L.）种类的序列同一性均为约83％。预计FtCHS的二级结构包含α-螺旋（41.73％），层（16.28％）和无规卷曲（41.98％）; FtCHS的三维建模的进一步研究包含11个α螺旋和15个β层，这表明蛋白质能够折叠成CHS蛋白质的典型三维结构，与MsCHS 2高度相似。预测的三级结构证明FtCHS具有结合位点。

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