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首页> 外文期刊>Russian Journal of Plant Physiology >Chalcone synthase homologous genes cloning and expression pattern in flowering Fagopyrum tataricum Gaertn
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Chalcone synthase homologous genes cloning and expression pattern in flowering Fagopyrum tataricum Gaertn

机译:苦荞麦中查尔酮合酶同源基因的克隆与表达模式

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摘要

Tartary buckwheat (Fagopyrum tataricum Gaertn.) is highly nutritious and an excellent dietary source of flavonoid compounds. Chalcone synthase (CHS) is the first key enzyme involved in flavonoid biosynthesis. Here, three putative CHS genes (designated as FtCHS1 (GU172165), FtCHS2 (KT284884), and FtCHS3 (KT284885) were isolated from tartary buckwheat. Nucleotide sequence analysis indicated that FtCHS1 and FtCHS2 each contained one intron of 444 bp and 157 bp, respectively. FtCHS3 included two introns, one of 86 bp and another of 73 bp. The results of quantitative real-time PCR (qRT-PCR) showed the FtCHSs expression presented the same pattern in the stems and flowers, with FtCHS1 > FtCHS3 > FtCHS2. A different tendency was found in leaves, with FtCHS3 > FtCHS2 > FtCHS1. However, there was no direct correlation between the three CHS expression and total flavonoids. Furthermore, high-performance liquid chromatography (HPLC) performance reveals rutin is the most abundant flavonoid in all tissues, leaves should be the main location for quercetin storage in tartary buckwheat.
机译:art荞麦(Fagopyrum tataricum Gaertn。)营养丰富,是类黄酮化合物的极佳饮食来源。查尔酮合酶(CHS)是类黄酮生物合成中的第一个关键酶。在此,从苦荞中分离出三个假定的CHS基因(分别命名为FtCHS1(GU172165),FtCHS2(KT284884)和FtCHS3(KT284885),核苷酸序列分析表明FtCHS1和FtCHS2分别包含一个444 bp和157 bp的内含子。 FtCHS3包含两个内含子,一个为86 bp,另一个为73 bp,定量实时PCR(qRT-PCR)结果显示FtCHSs在茎和花中的表达模式相同,FtCHS1> FtCHS3> FtCHS2。在叶片中发现了不同的趋势,即FtCHS3> FtCHS2> FtCHS1,但三种CHS的表达与总黄酮之间没有直接的相关性,而且高效液相色谱(HPLC)性能表明,芦丁是其中最丰富的类黄酮。所有组织,叶应该是苦荞中槲皮素储存的主要位置。

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