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首页> 外文会议>International conference on informatics and management science >Molecular Cloning and Sequences Analysis of Chalcone Synthase Gene from Fagopyrum Tataricum
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Molecular Cloning and Sequences Analysis of Chalcone Synthase Gene from Fagopyrum Tataricum

机译：来自豆类抑制型丘酮合成酶基因的分子克隆及序列分析

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One of the most important key enzymes Chalcone Synthase (CHS) gene was cloned from Fagopyrum tataricum. Based on cDNA sequence conserved domain of CHS, a pair of primers were designed and used to amplify a fragment of FtCHS gene (GenBank accession: EU715255) using RT-PCR and RACE technique. A 1,463 bp full-length cDNA sequence was obtained. Analysis of CHS cDNA indicated that it encoded a peptide containing 393 amino acids. The sequence identity to palm leaf rhubarb (Rheumpahlmatum L.) species was all about 83 %. It is predicted that secondary structure of FtCHS contains α-helix (41.73 %), layers (16.28 %) and random coils (41.98 %); further research on three-dimensional modeling of FtCHS contains 11 α-helixes and 15 β-layers, which showed that protein was able to fold to a typical three-dimensional structure of CHS protein highly similar to that of MsCHS 2. The predicted tertiary structure demonstrated that FtCHS has combination sites.
机译：来自Fagopyrum tataricum克隆的最重要的关键酶Chalcone合酶（CHS）基因之一。基于CHS的cDNA序列保守结构域，设计了一对引物，并使用RT-PCR和血液技术扩增FTCHS基因（Genbank ResciveS：Eu715255）的片段。获得1,463bp全长cDNA序列。 CHS cDNA分析表明它编码了含有393个氨基酸的肽。对棕榈叶大黄（Rheumpahlmatum L.）物种的序列同一性均为约83％。预测，FTCH的二级结构含有α-螺旋（41.73％），层（16.28％）和随机线圈（41.98％）;进一步研究FTCH的三维建模含有11α-螺旋和15β-层，其显示蛋白质能够折叠成高度相似的CHS蛋白的典型三维结构。预测的三级结构证明FTCH有组合网站。

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