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首页> 外文期刊>Developmental and Comparative Immunology: Ontogeny, Phylogeny, Aging: The Official Journal of the International Society of Developmental and Comparative Immunology >Hypoxia, reoxygenation and cytosolic manganese superoxide dismutase (cMnSOD) silencing in Litopenaeus vannamei: Effects on cMnSOD transcripts, superoxide dismutase activity and superoxide anion production capacity
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Hypoxia, reoxygenation and cytosolic manganese superoxide dismutase (cMnSOD) silencing in Litopenaeus vannamei: Effects on cMnSOD transcripts, superoxide dismutase activity and superoxide anion production capacity

机译：南美白对虾的缺氧，复氧和胞质锰超氧化物歧化酶（cMnSOD）沉默：对cMnSOD转录本，超氧化物歧化酶活性和超氧化物阴离子产生能力的影响

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摘要

The effects of silencing the mRNA of cytosolic manganese superoxide dismutase (cMnSOD), an enzyme involved in the antioxidant defense, were analyzed in Whiteleg shrimp, Litopenaeus vannamei adults. Shrimp were intramuscularly injected with long dsRNAs corresponding to the N-terminal portion of the cMnSOD and held under normoxic conditions for 24h. Another group of shrimp was exposed to hypoxia for 6h followed by reoxygenation for 1h. Shrimp injected with long dsRNAs had lower cMnSOD transcripts in gills and hepatopancreas. In the cMnSOD silenced shrimp, superoxide dismutase (SOD) activity decreased in gills but not in hepatopancreas. Shrimp subjected to hypoxia had lower cMnSOD transcripts and SOD activity in gills and hepatopancreas; the production of superoxide anion (O_2~-) by hemocytes was also lower in this group. Reoxygenation reverted the effect of hypoxia increasing the levels of cMnSOD transcripts, SOD activity and the production of O_2~-. These results suggest that cMnSOD contributes significantly to the SOD activity in gills and hepatopancreas and indicate its importance in the redox system regulation for L. vannamei.
机译：在南美白对虾（南美白对虾）成年虾中分析了沉默抗氧化防御酶胞质锰超氧化物歧化酶（cMnSOD）的mRNA的影响。虾肌肉内注射对应于cMnSOD N端部分的长dsRNA，并在常氧条件下保持24小时。另一组虾暴露于缺氧6h，然后再充氧1h。注射长dsRNA的虾在had和肝胰腺中的cMnSOD转录物较低。在cMnSOD沉默的虾中，g中的超氧化物歧化酶（SOD）活性降低，而肝胰腺中的却没有降低。缺氧的虾g和肝胰腺中的cMnSOD转录物和SOD活性较低。该组血细胞中超氧阴离子（O_2〜-）的产生也较低。复氧逆转了缺氧的作用，增加了cMnSOD转录水平，SOD活性和O_2〜-的产生。这些结果表明，cMnSOD显着促进了the和肝胰腺中SOD的活性，并表明其在南美白对虾氧化还原系统调节中的重要性。

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