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首页> 外文期刊>DTW:Deutsche Tierarztliche Wochenschrift >Effect of transport stress on the expression of heat shock protein 90 and its corresponding mRNA in the heart and the kidney of piglets
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Effect of transport stress on the expression of heat shock protein 90 and its corresponding mRNA in the heart and the kidney of piglets

机译：运输应激对仔猪心肾热休克蛋白90及其相关mRNA表达的影响。

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摘要

Transportation remains a major stressor for pigs leading to altered organ function and thereby affecting also product quality. The underlying molecular mechanisms remain to be clarified. Heat shock proteins (Hsps) are considered to counteract intracellular stress reactions. Therefore, the aim of this study was to investigate changes in the expression and localization of such a major defense molecule, namely Hsp90 in 2 h transported piglets. We analyzed Hsp90 at the protein and transcript level in theheart and kidney using qualitative and quantitative methods. Immunohistochemistry and in situ hybridization of Hsp90 identified the protein and mRNA in the cytoplasm and the nucleus of myocardial and renal tubular epithelial cells. The different localizations demonstrate the vital functions of Hsp90. Endothelial cells of both organs expressed high levels of Hsp90, which could be related to Hsp90 role in regulating blood flow. No obvious morphological changes of the Hsp90 distribution pattern could be identified in the cells of the transported piglet groups. However, using quantitative approaches, we identified a significant decrease of Hsp90 in the hearts at the protein (53.8% reduction, P < 0.05) and mRNA (45.3% reduction, P < 0.05) level. Hsp90 wasalso decreased in the kidney but did not reach a significant level. These results suggest that a short-term transport of 2 h is sufficient to decrease the amount of a vital stress defense molecule, which could explain transportation induced organ damage.
机译：运输仍然是猪的主要压力，导致器官功能改变，从而影响产品质量。潜在的分子机制仍有待阐明。热休克蛋白（Hsps）被认为可以抵消细胞内应激反应。因此，本研究的目的是研究运输2 h的仔猪中这种主要防御分子Hsp90的表达和定位的变化。我们使用定性和定量方法在心脏和肾脏的蛋白质和转录水平上分析了Hsp90。 Hsp90的免疫组织化学和原位杂交鉴定了心肌和肾小管上皮细胞的细胞质和细胞核中的蛋白质和mRNA。不同的位置展示了Hsp90的重要功能。两个器官的内皮细胞均表达高水平的Hsp90，这可能与Hsp90在调节血流中的作用有关。在运输的仔猪组的细胞中，没有发现Hsp90分布模式的明显形态变化。然而，使用定量方法，我们在蛋白质（降低53.8％，P <0.05）和mRNA（降低45.3％，P <0.05）水平上发现心脏中Hsp90的显着降低。 Hsp90在肾脏中也降低，但未达到显着水平。这些结果表明，短期运输2小时足以减少重要的应激防御分子的量，这可以解释运输引起的器官损伤。

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