...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Chemistry & biology >Profiling mechanisms of alkane hydroxylase activity in vivo using the diagnostic substrate norcarane
【24h】

Profiling mechanisms of alkane hydroxylase activity in vivo using the diagnostic substrate norcarane

机译：使用诊断底物正烷的体内烷烃羟化酶活性的分析机制

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Mechanistically informative chemical probes are used to characterize the activity of functional alkane hydroxylases in whole cells. Norcarane is a substrate used to reveal the lifetime of radical intermediates formed during alkane oxidation. Results from oxidations of this probe with organisms that contain the two most prevalent medium-chain-length alkane-oxidizing metalloenzymes, alkane omega-monooxygenase (AlkB) and cytochrome P450 (CYP), are reported. The results-radical lifetimes of 1-7 ns for AlkB and less than 100 ps for CYP-indicate that these two classes of enzymes are mechanistically distinguishable and that whole-cell mechanistic assays can identify the active hydroxylase. The oxidation of norcarane by several recently isolated strains (Hydrocarboni-phaga effusa AN 03, rJ4, and rJ5, whose alkane-oxidizing enzymes have not yet been identified) is also reported. Radical lifetimes of 1-3 ns are observed, consistent with these organisms containing an AlkB-like enzyme and inconsistent with their employing a CYP-like enzyme for growth on hydrocarbons.
机译：机械信息丰富的化学探针用于表征全细胞中功能性烷烃羟化酶的活性。正戊烷是用于揭示烷烃氧化过程中形成的自由基中间体寿命的底物。据报道，该探针被含有两种最普遍的中链烷氧基氧化金属酶，烷烃ω-单加氧酶（AlkB）和细胞色素P450（CYP）的生物体氧化的结果。结果表明，AlkB的自由基寿命为1-7 ns，而CYP的自由基寿命小于100 ps，表明这两类酶在机理上是可区分的，并且全细胞机理分析可以鉴定出活性羟化酶。还报道了几种最近分离的菌株（Hydrocarboni-phaga effusa AN 03，rJ4和rJ5，其烷烃氧化酶尚未被鉴定）对正二十烷的氧化。观察到1-3 ns的自由基寿命，这与这些含有AlkB样酶的生物一致，并且与使用CYP样酶在烃类上生长的生物不一致。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号