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首页> 外文期刊>Japanese Journal of Ophthalmology >Study of a polymerase chain reaction-based method for detection of herpes simplex virus type 1 DNA among Iranian patients with ocular herpetic keratitis infection
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Study of a polymerase chain reaction-based method for detection of herpes simplex virus type 1 DNA among Iranian patients with ocular herpetic keratitis infection

机译：基于聚合酶链反应的方法在伊朗眼疱疹性角膜炎感染患者中检测1型单纯疱疹病毒DNA的研究

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Purpose: To study the presence of the herpes simplex virus type 1 (HSV-1) glycoprotein D gene in tear films of Iranian patients with herpetic keratitis.Methods: Twenty-five tear film and eye swab specimens from 25 herpetic keratitis patients and 10 specimens from 10 healthy volunteers were collected in the Farabi Eye Hospital, Tehran, Iran. HSV-1 DNA was detected by using the nested polymerase chain reaction (nPCR) method. Viral isolation was done using conventional viral techniques. A monoclonal antibody-based enzyme-linked immunosorbent assay (ELISA) was used for confirmation of positive cytopathic effect cell culture. The results of a diagnosis by an ophthalmologist team were compared with those of nPCR.Results: HSV-1 DNA was identified in tear films of 88% (23/25) of suspected herpetic keratitis patients. All healthy controls (100%) had negative PCR results. HSV-1 was isolated in cell culture and confirmed by ELISA in 12% (3/25) of herpetic keratitis patients who had epithelial keratitis. The kappa value showed a high level of agreement between ophthalmologist team diagnosis and the PCR results (kappa = 0.86, P < 0.0001).Conclusions: nPCR is a sensitive, rapid, and powerful tool for detection of HSV-1 DNA in tear films of ocular herpetic keratitis patients and can serve as a supplemental method for diagnosis of herpetic keratitis infection. (C) Japanese Ophthalmological Society 2004
机译：目的：研究伊朗疱疹性角膜炎患者泪膜中单纯疱疹病毒1型（HSV-1）糖蛋白D基因的存在。方法：从25例疱疹性角膜炎患者和10例标本中提取25份泪膜和眼拭子样本。在伊朗德黑兰的法拉比眼科医院收集了来自10名健康志愿者的信息。使用巢式聚合酶链反应（nPCR）方法检测HSV-1 DNA。使用常规病毒技术进行病毒分离。基于单克隆抗体的酶联免疫吸附测定（ELISA）用于确认阳性细胞病变效应的细胞培养。将眼科医生团队的诊断结果与nPCR的结果进行比较。结果：在88％（23/25）的疑似疱疹性角膜炎患者的泪膜中鉴定出HSV-1 DNA。所有健康对照（100％）PCR均阴性。在细胞培养物中分离出HSV-1，并通过ELISA在12％（3/25）的上皮性角膜炎的疱疹性角膜炎患者中进行了确认。 kappa值表明眼科医生团队的诊断与PCR结果高度吻合（kappa = 0.86，P <0.0001）。结论：nPCR是检测泪膜中HSV-1 DNA的灵敏，快速且功能强大的工具。眼疱疹性角膜炎患者，可以作为诊断疱疹性角膜炎感染的补充方法。 （C）日本眼科学会2004

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